Recombinant Troponin I Type 3, Cardiac (TNNI3) | MyBioSource MBS2010502 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Troponin I Type 3, Cardiac (TNNI3)

catalog :

MBS2010502

quantity :

0.01 mg

price :

130 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2010502

products type :

Recombinant Protein

products full name :

Recombinant Troponin I Type 3, Cardiac (TNNI3)

products short name :

[Troponin I Type 3, Cardiac (TNNI3)]

other names :

[troponin I, cardiac muscle; Troponin I, cardiac muscle; troponin I, cardiac muscle; troponin I type 3 (cardiac); Cardiac troponin I]

products gene name :

[TNNI3]

other gene names :

[TNNI3; TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; TNNC1]

uniprot entry name :

TNNI3_HUMAN

host :

E. coli

reactivity :

Human

purity :

>98%

form :

10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.

concentration :

200µg/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

SDS-Page

other info1 :

Source: Prokaryotic expression. Residues: Met1~Gly203. Tags: N-terminal His-Tag. Tissue Specificity: Skeletal Muscle. Traits: Freeze-dried powder. Predicted isoelectric point: 9.7. Predicted Molecular Mass: 24.4kDa. Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

151101270

ncbi acc num :

NP_000354.4

ncbi gb acc num :

NM_000363.4

uniprot acc num :

P19429

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261)

ncbi summary :

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

uniprot summary :

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family. Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: sarcomere; troponin complex; cytosol. Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding. Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction. Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a

size1 :

0.01 mg

price1 :

130 USD

size2 :

0.05 mg

price2 :

225

size3 :

0.1 mg

price3 :

340

size4 :

0.2 mg

price4 :

415

size5 :

0.5 mg

price5 :

800

size6 :

1 mg

price6 :

1180

more info or order :

MyBioSource product webpage