Recombinant Tumor Necrosis Factor Beta (TNFb) | MyBioSource MBS2010440 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Tumor Necrosis Factor Beta (TNFb)

catalog :

MBS2010440

quantity :

0.01 mg

price :

175 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010440

products type :

Recombinant Protein

products full name :

Recombinant Tumor Necrosis Factor Beta (TNFb)

products short name :

Tumor Necrosis Factor Beta (TNFb)

other names :

lymphotoxin-alpha; Lymphotoxin-alpha; lymphotoxin-alpha; TNF beta; lymphotoxin alpha; tumor necrosis factor ligand superfamily member 1; lymphotoxin A; TNF-beta; Tumor necrosis factor ligand superfamily member 1

products gene name :

TNFb

other gene names :

Lta; Lta; LT; Ltx; Tnfb; LT[a]; LT-[a]; TNFSF1; hlb382; LTalpha; Tnfsf1b; LT-alpha; TNF-beta; Tnfb; Tnfsf1; LT-alpha

uniprot entry name :

TNFB_MOUSE

host :

E Coli

reactivity :

Mus musculus (Mouse)

sequence length :

202

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF- PQ KHLTHGILKP AAHLVGYPSK QNSLLWRAST DRAFLRHGFS LSNNSLLIPT SGLYFVYSQV VFSGESCSPR AIPTPIYLAH EVQLFSSQYP FHVPLLSAQK SVYPGLQGPW VRSMYQGAVF LLSKGDQLST HTDGISHLHF SPSSVFFGAF AL

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). (May be suitable for use in other assays to be determined by the end user.)

other info1 :

Expression System: Prokaryotic expression. Residues: Pro49~Leu202 (Accession # P09225) with N-terminal His-Tag. Predicted isoelectric point: 9.4

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

7106343

ncbi acc num :

NP_034865.1

ncbi gb acc num :

NM_010735.2

uniprot acc num :

P09225

ncbi mol weight :

18.5kDa

ncbi pathways :

Apoptosis Pathway (198339); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865); NF-kappa B Signaling Pathway (634537); TNF Signaling Pathway (812263); TNF Signaling Pathway (813210)

uniprot summary :

LTA: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the tumor necrosis factor family. Protein type: Apoptosis; Secreted, signal peptide; Cytokine; Secreted. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding. Biological Process: positive regulation of humoral immune response mediated by circulating immunoglobulin; transformed cell apoptosis; positive regulation of apoptosis; lymph node development; humoral immune response; cell proliferation; positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; negative regulation of fibroblast proliferation; positive regulation of cell proliferation; positive regulation of chronic inflammatory response to antigenic stimulus; immune response; inflammatory response; cellular process

size1 :

0.01 mg

price1 :

175 USD

size2 :

0.05 mg

price2 :

360

size3 :

0.1 mg

price3 :

560

more info or order :

MyBioSource product webpage