Recombinant Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) | MyBioSource MBS2010388 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1)

catalog :

MBS2010388

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010388

products type :

Recombinant Protein

products full name :

Recombinant Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1)

products short name :

Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1)

other names :

NADPH oxidase 1 isoform 3; NADPH oxidase 1; NADPH oxidase 1; mitogenic oxidase 1; NADPH oxidase homolog-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); NADPH oxidase 1; Mitogenic oxidase 1; MOX-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NOH-1

products gene name :

NOX1

other gene names :

NOX1; NOX1; MOX1; NOH1; NOH-1; GP91-2; MOX1; NOH1; NOX-1; MOX-1

uniprot entry name :

NOX1_HUMAN

host :

E Coli

sequence length :

527

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF- MNESHP RKCAESFEMW DDRDSHCRRP KFEGHPPESW KWILAPVILY ICERILRFYR SQQKVVITKV VMHPSKVLEL QMNKRGFSME VGQYIFVNCP SISLLEWHPF TLTSAPEEDF FSIHIRAAGD WTENLIRAFE QQYSPIPRIE VDGPFGTASE DVFQYEVAVL VGAGIGVTPF ASILKSIWYK FQCADHNLKT KKIYFYWICR ETGAFSWFNN LLTSLEQEME ELGKVGFLNY RLFLTGWDSN IVGHAALN

purity :

> 98%

form :

20mM Tris, 150mM NaCI, pH8.0, containing 1mM EDTA, 1 mM OTT, 0.01 % sarcosyl, 5% Trehalose and Proclin300

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)!!IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Met235~Asn488 with N-terminal His-Tag. Subcellular Location: Invadopodium membrane. Tissue Specificity: Brain, Liver, Heart, Kidney. Traits: Freeze-dried powder

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted isoelectric point: 6.3. Predicted Molecular Mass: 31.1 kDa. Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCI (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

425854820

ncbi acc num :

NP_001258744.1

ncbi gb acc num :

NM_001271815.1

uniprot acc num :

Q9Y5S8

ncbi pathways :

HIF-1 Signaling Pathway (695200); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); Phagosome Pathway (153910); Phagosome Pathway (153859); RAC1 Signaling Pathway (169355)

ncbi summary :

This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

uniprot summary :

NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Channel, misc.; Oxidoreductase; EC 1.-.-.-; Motility/polarity/chemotaxis; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: Xq22. Cellular Component: early endosome; integral to membrane; cell junction; NADPH oxidase complex. Molecular Function: protein binding; metal ion binding; superoxide-generating NADPH oxidase activity; Rac GTPase binding; NADP binding; voltage-gated proton channel activity. Biological Process: intracellular pH elevation; respiratory burst; extracellular matrix organization and biogenesis; cell migration; positive regulation of smooth muscle cell proliferation; superoxide metabolic process; positive regulation of JNK cascade; signal transduction; regulation of systemic arterial blood pressure by renin-angiotensin; NADP metabolic process; proton transport; regulation of blood pressure; positive regulation of cell proliferation; hydrogen peroxide metabolic process; positive regulation of integrin biosynthetic process; angiogenesis; inflammatory response; superoxide release

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

395

size3 :

0.1 mg

price3 :

610

more info or order :

MyBioSource product webpage