Recombinant Perforin 1 (PRF1) | MyBioSource MBS2010346 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Perforin 1 (PRF1)

catalog :

MBS2010346

quantity :

0.01 mg

price :

220 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010346

products type :

Recombinant Protein

products full name :

Recombinant Perforin 1 (PRF1)

products short name :

Perforin 1 (PRF1)

other names :

perforin-1; Perforin-1; perforin-1; P1; cytolysin; pore forming protein; lymphocyte pore-forming protein; perforin 1 (pore forming protein); Cytolysin; Lymphocyte pore-forming protein

products gene name :

PRF1

other gene names :

Prf1; Prf1; Pfn; Pfp; Prf-1; Pfp; P1

uniprot entry name :

PERF_MOUSE

host :

E Coli

sequence length :

554

sequence :

The sequence of the target protein is listed below. V WMAGEGMDVT TLRRSGSFPV NTQRFLRPDR TCTLCKNSLM RDATQRLPVA ITHWRPHSSH CQRNVAAAKV HSTEGVAREA AANINNDWRV GLDVNPRPEA NMRASVAGSH SKVANFAAEK TYQDQYNFNS DTVECRMYSF RLVQKPPLHL DFKKALRALP RNFNSSTEHA YHRLISSYGT HFITAVDLGG RISVLTALRT CQLTLNGLTA DEVGDCLNVE AQVSIGAQAS VSSEYKACEE KKKQHKMATS FHQTYRERHV EVLGGPLDST HDLLFGNQAT PEQFSTWTAS LPSNPGLVDY SLEPLHTLLE EQNPK

purity :

> 95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 0.1% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). (May be suitable for use in other assays to be determined by the end user.)

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Val40-Lys355 (Accession # P10820) with two N-terminal Tags, His-tag and GST-tag. Subcellular Location: Cytoplasmic granule lumen. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 6.8. Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

225735600

ncbi acc num :

NP_035203.3

ncbi gb acc num :

NM_011073.3

uniprot acc num :

P10820

ncbi mol weight :

67.2kDa

ncbi pathways :

Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Apoptosis Pathway (198339); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); Graft-versus-host Disease Pathway (83317); Graft-versus-host Disease Pathway (536); Natural Killer Cell Mediated Cytotoxicity Pathway (83276); Natural Killer Cell Mediated Cytotoxicity Pathway (490); Type I Diabetes Mellitus Pathway (83292)

uniprot summary :

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family. Protein type: Membrane protein, multi-pass. Cellular Component: extracellular space; membrane; cytoplasmic membrane-bound vesicle; plasma membrane; extracellular region; integral to membrane; cytoplasmic vesicle; endosome. Molecular Function: metal ion binding; calcium ion binding; wide pore channel activity. Biological Process: circadian rhythm; formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; immune response to tumor cell; transmembrane transport; defense response to virus; protein homooligomerization

size1 :

0.01 mg

price1 :

220 USD

size2 :

0.05 mg

price2 :

485

size3 :

0.1 mg

price3 :

755

more info or order :

MyBioSource product webpage