protein

Recombinant Slit Homolog 3 (Slit3)

MBS2010323

0.01 mg

135 USD

Recombinant Protein

Recombinant Slit Homolog 3 (Slit3)

[Slit Homolog 3 (Slit3)]

[slit homolog 3 protein; Slit homolog 3 protein; slit homolog 3 protein; slit-3; slit homolog 3 (Drosophila)]

[Slit3]

[Slit3; Slit3; Slil2; Slit1; Slit-3; Slit3]

SLIT3_MOUSE

E. coli

Mouse

>98%

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarkosyl, 5%Trehalose and Proclin300.

200ug/ml

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 Degee C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 Degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Source: Prokaryotic expression. Residues: Lys1348~Cys1517. Tags: N-terminal His-Tag. Subcellular Location: Secreted. Traits: Freeze-dried powder. Original Concentration: 200µg/mL

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Predicted isoelectric point: 6.0. Predicted Molecular Mass: 24.3kDa. Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows: . 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

226823283

NP_035542.2

NM_011412.3

Q9WVB4

Axon Guidance Pathway (83262); Axon Guidance Pathway (476); Axon Guidance Pathway (926383); Developmental Biology Pathway (926382); Netrin-1 Signaling Pathway (926394); Spinal Cord Injury Pathway (739004)

SLIT3: May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; mitochondrion; membrane; extracellular region. Molecular Function: Roundabout binding; calcium ion binding; receptor binding. Biological Process: axon extension involved in axon guidance; nervous system development; axon guidance; negative regulation of cell proliferation; organ morphogenesis; multicellular organismal development; negative chemotaxis; negative regulation of cell growth; cell differentiation

0.01 mg

135 USD

0.05 mg

245

0.1 mg

365

0.2 mg

450

0.5 mg

870

1 mg

1280