protein

Recombinant Dickkopf Related Protein 4 (DKK4)

MBS2010241

0.01 mg

205 USD

Recombinant Protein

Recombinant Dickkopf Related Protein 4 (DKK4)

Dickkopf Related Protein 4 (DKK4)

dickkopf-related protein 4; Dickkopf-related protein 4; dickkopf-related protein 4; dickkopf-4; dickkopf homolog 4 (Xenopus laevis)

DKK4

Dkk4; Dkk4; Dkk-4; Dickkopf-4; Dkk-4

DKK4_MOUSE

E Coli

221

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-KGSL CASDRDCSEG KFCLAFHDERSFCATCRRVR RRCQRSAVCC PGTVCVNDVC TAVEDTRPVM DRNTDGQDGA YAEGTTKWPA EENRPQGKPS TKKSQSSKGQ E

> 95%

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Lys37~Glu141 (Accession # Q8VEJ3) with two N-terminal Tags, His-tag and GST-tag

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted Isoelectric Point: 6.5. Predicted Molecular Mass: 43.5kDa. Accurate Molecular Mass: 50kDa as determined by SDS-PAGE reducing conditions. Storage Buffer: 62.5mM Tris-H3PO4 (pH 7.5 at 25 degree C), 1mM EDTA, 2% (w/v) SDS, 100mM DTT, 1mM NaN3, 0.01% (w/v) bromo-phenol blue and 33% (v/v) glycerol.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

21704186

NP_663567.1

NM_145592.2

Q8VEJ3

Wnt Signaling Pathway (83258); Wnt Signaling Pathway (471)

DKK4: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family. Protein type: Secreted; Secreted, signal peptide; Inhibitor. Cellular Component: extracellular region. Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; multicellular organismal development

0.01 mg

205 USD

0.05 mg

450

0.1 mg

700