catalog number :
MBS2010182
products type :
Recombinant Protein
products full name :
Recombinant Proteinase 3 (PR3)
products short name :
Proteinase 3 (PR3)
other names :
myeloblastin; Myeloblastin; myeloblastin; PR-3; proteinase 3; Proteinase 3
other gene names :
Prtn3; Prtn3; PR3; mPR3; PR-3
uniprot entry name :
PRTN3_MOUSE
sequence :
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-AVQASKIVGG HEARPHSRPY VASLQLSRFP GSHFCGGTLI HPRFVLTAAH CLQDISWQLV TVVLGAHDLL SSEPEQQKFT ISQVFQNNYN PEENLNDVLL LQLNRTASLG KEVAVASLPQ QDQTLSQGTQ CLAMGWGRLG TQAPTPRVLQ ELNVTVVTFL CREHNVCTLV PRRAAGICFG DSGGPLICNG ILHGVDSFVI RECASLQFPD FFARVSMYVD WIQNVL
form :
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Ala24~Leu249 (Accession # Q61096) with N-terminal His-Tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in ddH2O. Predicted isoelectric point: 6.5. Predicted Molecular Mass: 26.3kDa
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_035308.2
ncbi gb acc num :
NM_011178.2
uniprot summary :
PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily. Protein type: Cell cycle regulation; Protease; EC 3.4.21.76. Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol. Molecular Function: peptidase activity; enzyme binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity. Biological Process: collagen catabolic process; negative regulation of phagocytosis; proteolysis