Recombinant Fibulin 1 (FBLN1) | MyBioSource MBS2010143 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Fibulin 1 (FBLN1)

catalog :

MBS2010143

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2010143

products type :

Recombinant Protein

products full name :

Recombinant Fibulin 1 (FBLN1)

products short name :

[Fibulin 1 (FBLN1)]

other names :

[fibulin-1 isoform C; Fibulin-1; fibulin-1; fibulin 1]

products gene name :

[FBLN1]

other gene names :

[FBLN1; FBLN1; FBLN; FIBL1; FIBL-1]

uniprot entry name :

FBLN1_HUMAN

host :

E Coli

reactivity :

Homo sapiens (Human)

sequence length :

683

sequence :

The sequence of the target protein is listed below. LNDRC RGGGPCKQQC RDTGDEVVCS CFVGYQLLSD GVSCEDVNEC ITGSHSCRLG ESCINTVGSF RCQRDSSCGT GYELTEDNSC KDIDECESGI HNCLPDFICQ NTLGSFRCRP KLQCKSGFIQ DALGNCIDIN ECLSISAPCP IGHTCINTEG SYTCQKNVPN CGRGYHLNEE GTRCVDVDEC APPAEPCGKG HRCVNSPGSF RCECKTGYYF DGISRMCV

purity :

> 95%

form :

Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

image1 heading :

SDS-Page

other info1 :

Residues: Asp399~Ile578 (Accession # P23142) with N-terminal His-Tag. Subcellular Location: Secreted, extracellular space, extracellular matrix

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted isoelectric point: 5.4. Predicted Molecular Mass: 25.7kDa. Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions. Reconstitution: Reconstitute in sterile ddH2O.

ncbi gi num :

34734062

ncbi acc num :

NP_001987.2

ncbi gb acc num :

NM_001996.3

uniprot acc num :

P23142

ncbi pathways :

Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Molecules Associated With Elastic Fibres Pathway (730311)

ncbi summary :

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]

uniprot summary :

FBLN1: Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation. Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane. Molecular Function: protease activator activity; extracellular matrix structural constituent; calcium ion binding. Biological Process: extracellular matrix organization and biogenesis; viral reproduction; embryo implantation. Disease: Synpolydactyly 2

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

260

size3 :

0.1 mg

price3 :

395

size4 :

0.2 mg

price4 :

485

size5 :

0.5 mg

price5 :

945

size6 :

1 mg

price6 :

1395

more info or order :

MyBioSource product webpage