Recombinant Low Density Lipoprotein Receptor Related Protein 5 (LRP5) | MyBioSource MBS2010133 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Low Density Lipoprotein Receptor Related Protein 5 (LRP5)

catalog :

MBS2010133

quantity :

0.01 mg

price :

195 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010133

products type :

Recombinant Protein

products full name :

Recombinant Low Density Lipoprotein Receptor Related Protein 5 (LRP5)

products short name :

Low Density Lipoprotein Receptor Related Protein 5 (LRP5)

other names :

low-density lipoprotein receptor-related protein 5; Low-density lipoprotein receptor-related protein 5; low-density lipoprotein receptor-related protein 5; LRP-5; low density lipoprotein receptor-related protein 7; low density lipoprotein receptor-related protein 5

products gene name :

LRP5

other gene names :

LRP5; LRP5; HBM; LR3; OPS; EVR1; EVR4; LRP7; OPPG; BMND1; OPTA1; VBCH2; LR3; LRP7; LRP-5

uniprot entry name :

LRP5_HUMAN

host :

E. coli

reactivity :

Homo sapiens (Human)

sequence length :

1615

purity :

> 95%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

other info1 :

Source: Prokaryotic expression. Residues: Asp769~Leu1016 . Tags: N-terminal His-Tag. Tissue Specificity: Liver, Aorta. Subcellular Location: Membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Traits: Freeze-dried powder

other info2 :

Predicted isoelectric point: 6.2. Predicted Molecular Mass: 31.9kDa. Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

119709832

ncbi acc num :

NP_002326.2

ncbi gb acc num :

NM_002335.2

uniprot acc num :

O75197

ncbi pathways :

MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); N-cadherin Signaling Events Pathway (137919); Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway And Pluripotency (198847); Wnt Signaling Network Pathway (137962); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)

ncbi summary :

This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

uniprot summary :

LRP5: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts with FZD8; the interaction is formed on WNT-binding and signaling. Interacts (via the phosphorylated PPPSP motif domains) with AXIN1; the interaction prevents inhibition of beta-catenin phosphorylation and signaling and is enhanced in the presence of GSK3B and WNT1 or WNT3A. Interacts (via beta-propeller regions 3 and 4) with DKK1; the interaction, enhanced by MESD and/or KREMEN, inhibits beta-catenin signaling by preventing GSK3-mediated phosphorylation of the PPPSP motifs and subsequent, AXIN1 binding. Interacts with MESD; the interaction prevents the formation of LRP5 aggregates, targets LRP5 to the plasma membrane and, when complexed with KREMEN2, increases DKK1 binding. Interacts with CSNK1E. Interacts with SOST; the interaction antagonizes canonical Wnt signaling. Interacts with APCDD1. Widely expressed, with the highest level of expression in the liver and in aorta. Belongs to the LDLR family. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 11q13.4. Cellular Component: mitochondrion; endoplasmic reticulum; integral to membrane; plasma membrane; receptor complex. Molecular Function: Wnt-protein binding; Wnt receptor activity; protein binding; toxin transporter activity; coreceptor activity. Biological Process: response to peptide hormone stimulus; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; bone remodeling; anatomical structure regression; anterior/posterior pattern formation; regulation of apoptosis; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; negative regulation of osteoblast differentiation; cell migration involved in gastrulation; embryonic limb morphogenesis; cholesterol metabolic process; Wnt receptor signaling pathway; positive regulation of mitosis; regulation of bone remodeling; endocytosis; gastrulation with mouth forming second; osteoblast development; bone marrow development; glucose catabolic process; cholesterol homeostasis; embryonic retina morphogenesis in camera-type eye; positive regulation of osteoblast proliferation; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; retina morphogenesis in camera-type eye. Disease: Van Buchem Disease, Type 2; Osteoporosis-pseudoglioma Syndrome; Osteopetrosis, Autosomal Dominant 1; Exudative Vitreoretinopathy 1; Exudative Vitreoretinopathy 4; Bone Mineral Density Quantitative Trait Locus 1; Osteoporosis; Endosteal Hyperostosis, Autosomal Dominant

size1 :

0.01 mg

price1 :

195 USD

size2 :

0.05 mg

price2 :

410

size3 :

0.1 mg

price3 :

635

more info or order :

MyBioSource product webpage