Recombinant Neutral Sphingomyelinase (NSMASE) | MyBioSource MBS2010016 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Neutral Sphingomyelinase (NSMASE)

catalog :

MBS2010016

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010016

products type :

Recombinant Protein

products full name :

Recombinant Neutral Sphingomyelinase (NSMASE)

products short name :

Neutral Sphingomyelinase (NSMASE)

other names :

sphingomyelin phosphodiesterase 2; Sphingomyelin phosphodiesterase 2; sphingomyelin phosphodiesterase 2; N-SMase; lyso-PAF-PLC; neutral sphingomyelinase; lyso-platelet-activating factor-phospholipase C; sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase); Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase

products gene name :

NSMASE

other gene names :

SMPD2; SMPD2; ISC1; NSMASE; NSMASE1; Lyso-PAF-PLC; N-SMase; nSMase

uniprot entry name :

NSMA_HUMAN

host :

E Coli

sequence length :

423

sequence :

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-KPNFSLRLR IFNLNCWGIP YLSKHRADRM RRLGDFLNQE SFDLALLEEV WSEQDFQYLRQKLSPTYPAA HHFRSGIIGS GLCVFSKHPI QELTQHIYTL NGYPYMIHHG DWFSGKAVGL LVLHLSGMVL NAYVTHLHAE YNRQKDIYLA HRVAQAWELA QFIHHTSKKA DVVLLCGDLN MHPEDLGCCL LKEWTGLHDA YLETRDFKGS EEGNTMVPKN CYVSQQELKP FPFGVR

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% Sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Lys2-Arg236 (Accession # O60906) with two N-terminal Tags, His-tag and GST-tag. Predicted isoelectric point: 6.6

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

92859618

ncbi acc num :

NP_003071.2

ncbi gb acc num :

NM_003080.2

uniprot acc num :

O60906

ncbi mol weight :

59.2kDa

ncbi pathways :

Ceramide Signalling Pathway (106451); Glycosphingolipid Metabolism Pathway (530751); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Signal Transduction Pathway (477114); Signalling By NGF Pathway (106439); Sphingolipid Metabolism Pathway (82994); Sphingolipid Metabolism Pathway (119543); Sphingolipid Metabolism Pathway (369); TNF Receptor Signaling Pathway (138053)

ncbi summary :

This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009]

uniprot summary :

SMPD2: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. Belongs to the neutral sphingomyelinase family. Protein type: Phosphodiesterase; Membrane protein, integral; Membrane protein, multi-pass; EC 3.1.4.12; Lipid Metabolism - sphingolipid. Chromosomal Location of Human Ortholog: 6q21. Cellular Component: integral to plasma membrane; plasma membrane; caveola. Molecular Function: sphingomyelin phosphodiesterase activity; metal ion binding. Biological Process: sphingomyelin metabolic process; sphingolipid metabolic process; nerve growth factor receptor signaling pathway; response to mechanical stimulus; ceramide biosynthetic process; glycosphingolipid metabolic process

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

405

size3 :

0.1 mg

price3 :

625

more info or order :

MyBioSource product webpage