Recombinant Neprilysin (NEP) | MyBioSource MBS2009960 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Neprilysin (NEP)

catalog :

MBS2009960

quantity :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2009960

products type :

Recombinant Protein

products full name :

Recombinant Neprilysin (NEP)

products short name :

Neprilysin (NEP)

other names :

neprilysin; Neprilysin; neprilysin; enkephalinase; atriopeptidase; skin fibroblast elastase; neutral endopeptidase 24.11; membrane metallo endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase); membrane metallo-endopeptidase; Atriopeptidase; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase

products gene name :

NEP

other gene names :

Mme; Mme; Nep; SFE; CD10; NEP; Neutral endopeptidase; SFE

uniprot entry name :

NEP_RAT

host :

E Coli

sequence length :

750

sequence :

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-ATTKP EDRNDPMLLY NKMTLAKLQN NFSLEINGKP FSWSNFTNEI MSTVNINIQN EEEVVVYAPE YLTKLKPILT KYSPRDLQNL MSWRFIMDLV SSLSRNYKES RNAFRKALYG TTSETATWRR CANYVNGNME NAVGRLYV

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) . (May be suitable for use in other assays to be determined by the end user.)

other info1 :

Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Ala286~Val428 (Accession # P07861), with two N-terminal Tags, His-tag and GST-tag.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted isoelectric point: 7.2. Predicted Molecular Mass: 49.0 kDa. USAGE: Reconstitute in ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

6981210

ncbi acc num :

NP_036740.1

ncbi gb acc num :

NM_012608.2

uniprot acc num :

P07861

ncbi mol weight :

18.2kDa

ncbi pathways :

Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Hematopoietic Cell Lineage Pathway (83470); Hematopoietic Cell Lineage Pathway (489); Metabolism Of Angiotensinogen To Angiotensins Pathway (935857); Metabolism Of Proteins Pathway (935800); Peptide Hormone Metabolism Pathway (935854); Protein Digestion And Absorption Pathway (172816); Protein Digestion And Absorption Pathway (171868); Renin - Angiotensin System Pathway (219775)

ncbi summary :

zinc-containing metallo-enzyme; involved in degradation of cardiac peptides [RGD, Feb 2006]

uniprot summary :

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family. Protein type: Membrane protein, integral; EC 3.4.24.11; Protease. Cellular Component: synaptic vesicle; focal adhesion; membrane; axon; dendrite; cytoplasm; plasma membrane; integral to membrane; synapse; brush border. Molecular Function: peptidase activity; zinc ion binding; metalloendopeptidase activity; endopeptidase activity; exopeptidase activity; peptide binding. Biological Process: peptide metabolic process; beta-amyloid metabolic process; creatinine metabolic process; proteolysis; kidney development; sensory perception of pain

size1 :

0.01 mg

price1 :

205 USD

size2 :

0.05 mg

price2 :

455

size3 :

0.1 mg

price3 :

710

more info or order :

MyBioSource product webpage