catalog number :
MBS2009901
products type :
Recombinant Protein
products full name :
Recombinant Prostate Stem Cell Antigen (PSCA)
products short name :
Prostate Stem Cell Antigen (PSCA)
other names :
Prostate stem cell antigen; Prostate stem cell antigen
products gene name :
PSCA
uniprot entry name :
PSCA_HUMAN
sequence :
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-LCYSCKAQV SNEDCLQVEN CTQLGEQCWT ARIRAVGLLT VISKGCSLNC VDDSQDYYVG KKNITCCDTD LCNAS
form :
Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Leu22~Ser95 (Accession # O43653) with N-terminal His-Tag. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 5.8. Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted Molecular Mass: 9.6kDa
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi mol weight :
15kDa as determined by SDS-PAGE reducing conditions.
uniprot summary :
PSCA: May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 8q24.2. Cellular Component: plasma membrane