protein

Recombinant Sulfatase 2 (SULF2)

MBS2009896

0.01 mg

150 USD

Recombinant Protein

Recombinant Sulfatase 2 (SULF2)

[Sulfatase 2 (SULF2)]

[extracellular sulfatase Sulf-2 isoform a; Extracellular sulfatase Sulf-2; extracellular sulfatase Sulf-2; sulfatase 2]

[SULF2]

[SULF2; SULF2; HSULF-2; KIAA1247; hSulf-2]

SULF2_HUMAN

E Coli

> 95%

Original Concentration: 200 Ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Phe337~Arg649. Tags: N-terminal His and GST Tag. Subcellular Location: Golgi apparatus. Traits: Freeze-dried powder. Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Predicted isoelectric point: 8.7. Predicted Molecular Mass: 65.7kDa. Accurate Molecular Mass: 65kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

240255483

NP_001155313.1

NM_001161841.1

Q8IWU5

Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]

SULF2: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; EC 3.1.6.-; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular space; Golgi stack; cell surface; endoplasmic reticulum; plasma membrane. Molecular Function: arylsulfatase activity; N-acetylglucosamine-6-sulfatase activity; calcium ion binding. Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; embryonic skeletal development; cartilage development; glomerular filtration; glomerular basement membrane development; heparan sulfate proteoglycan metabolic process; esophagus smooth muscle contraction; chondrocyte development; kidney development; positive regulation of Wnt receptor signaling pathway

0.01 mg

150 USD

0.05 mg

285

0.1 mg

445

0.2 mg

550

0.5 mg

1060

1 mg

1580