protein

Recombinant Mucin 4 (MUC4)

MBS2009865

0.01 mg

200 USD

Recombinant Protein

Recombinant Mucin 4 (MUC4)

Mucin 4 (MUC4)

mucin-4 isoform d; Mucin-4; mucin-4; testis mucin; tracheobronchial mucin; ascites sialoglycoprotein; mucin 4, tracheobronchial; pancreatic adenocarcinoma mucin; mucin 4, cell surface associated; Ascites sialoglycoprotein; ASGP; Pancreatic adenocarcinoma mucin; Testis mucin; Tracheobronchial mucinCleaved into the following 2 chains:Mucin-4 alpha chain; Alternative name(s):; Ascites sialoglycoprotein 1; ASGP-1Mucin-4 beta chain; Alternative name(s):; Ascites sialoglycoprotein 2

MUC4

MUC4; MUC4; ASGP; MUC-4; HSA276359; MUC-4; ASGP; ASGP-1; ASGP-2

MUC4_HUMAN

E Coli

1176

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGS-PFWDDAD FSTGRGTTFY QEYETFYGEH SLLVQQAESW IRKMTNNGGY KARWALKVTW VNAHAYPAQW TLGSNTYQAI LSTDGSRSYA LFLYQSGGMQ WDVAQRSGNP VLMGFSSGDG YFENSPLMSQ PVWERYRPDR FLNSNSGLQG LQFYRLHRE

> 90%

Supplied as lyophilized form in 10mM PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Pro1154~Glu1309 (Accession # Q99102) with N-terminal His-Tag. Subcellular Location: Cell membrane; Single-pass membrane protein, Secreted. Predicted isoelectric point: 6.8. Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Note: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice Variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affect the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation clevage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

112382233

NP_004523.3

NM_004532.5

Q99102

19.3kDa

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number ( 100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008]

MUC4: May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 Tyr-1248 . In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. 14 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q29. Cellular Component: extracellular space; proteinaceous extracellular matrix; membrane; Golgi lumen; integral to plasma membrane; vesicle. Molecular Function: ErbB-2 class receptor binding; extracellular matrix constituent, lubricant activity. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; maintenance of gastrointestinal epithelium; cell-matrix adhesion; post-translational protein modification

0.01 mg

200 USD

0.05 mg

430

0.1 mg

665