Recombinant Cytochrome P450 2D6 (CYP2D6) | MyBioSource MBS2009858 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Cytochrome P450 2D6 (CYP2D6)

catalog :

MBS2009858

quantity :

0.01 mg

price :

185 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2009858

products type :

Recombinant Protein

products full name :

Recombinant Cytochrome P450 2D6 (CYP2D6)

products short name :

Cytochrome P450 2D6 (CYP2D6)

other names :

cytochrome P450 2D6 isoform 1; Cytochrome P450 2D6; cytochrome P450 2D6; cytochrome P450-DB1; microsomal monooxygenase; xenobiotic monooxygenase; debrisoquine 4-hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, family 2, subfamily D, polypeptide 7 pseudogene 2; cytochrome P450, family 2, subfamily D, polypeptide 8 pseudogene 2; cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing)-like 1; cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing), polypeptide 6; cytochrome P450, subfamily II (debrisoquine, sparteine, etc., -metabolising), polypeptide 7 pseudogene 2; cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolising), polypeptide 8 pseudogene 2; cytochrome P450, family 2, subfamily D, polypeptide 6; CYPIID6; Cytochrome P450-DB1; Debrisoquine 4-hydroxylase

products gene name :

CYP2D6

other gene names :

CYP2D6; CYP2D6; CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1; CYP2DL1

uniprot entry name :

CP2D6_HUMAN

host :

E Coli

sequence length :

497

sequence :

The sequence of the target protein is listed below. LAGKV LRFQKAFLTQ LDELLTEHRM TWDPAQPPRD LTEAFLAEME KAKGNPESSF NDENLRIVVA DLFSAGMVTT STTLAWGLLL MILHPDVQRR VQQEIDDVIG QVRRPEMGDQ AHMPYTTAVI HEVQRFGDIV PLGVTHMTSR DIEVQGFRIP KGTTLITNLS SVLKDEAVWE KPFRFHPEHF LDAQGHFVKP EAFLPFSAGR RACLGEPLAR MELFLFFTSL LQHFSFSVPT GQ

purity :

>90%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). (May be suitable for use in other assays to be determined by the end user.)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Leu236~Gln472 with N-terminal His-Tag. Usage: Reconstitute in ddH2O. Accession #: P10635. Predicted isoelectric point: 6.0

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

392513721

ncbi acc num :

NP_000097.3

ncbi gb acc num :

NM_000106.5

uniprot acc num :

P10635

ncbi mol weight :

28.4kDa

ncbi pathways :

Biological Oxidations Pathway (105698); CYP2E1 Reactions Pathway (105705); Codeine And Morphine Metabolism Pathway (198831); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Fatty Acid Omega Oxidation Pathway (198915); Fatty Acids Pathway (105706); Metabolism Pathway (477135); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 25% of commonly prescribed drugs. Its substrates include antidepressants, antipsychotics, analgesics and antitussives, beta adrenergic blocking agents, antiarrythmics and antiemetics. The gene is highly polymorphic in the human population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Some individuals with the poor metabolizer phenotype have no functional protein since they carry 2 null alleles whereas in other individuals the gene is absent. This gene can vary in copy number and individuals with the ultrarapid metabolizer phenotype can have 3 or more active copies of the gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

uniprot summary :

CYP2D6: Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. Belongs to the cytochrome P450 family. Protein type: Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.14.1. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum. Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; drug binding; oxidoreductase activity; oxygen binding; monooxygenase activity. Biological Process: steroid metabolic process; coumarin metabolic process; monoterpenoid metabolic process; isoquinoline alkaloid metabolic process; negative regulation of binding; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; alkaloid metabolic process; alkaloid catabolic process; heterocycle metabolic process; drug metabolic process. Disease: Drug Metabolism, Poor, Cyp2d6-related

size1 :

0.01 mg

price1 :

185 USD

size2 :

0.05 mg

price2 :

385

size3 :

0.1 mg

price3 :

595

more info or order :

MyBioSource product webpage