Recombinant Neurofilament, Light Polypeptide (NEFL) | MyBioSource MBS2009843 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Neurofilament, Light Polypeptide (NEFL)

catalog :

MBS2009843

quantity :

0.01 mg

price :

135 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2009843

products type :

Recombinant Protein

products full name :

Recombinant Neurofilament, Light Polypeptide (NEFL)

products short name :

[Neurofilament, Light Polypeptide (NEFL)]

other names :

[neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein]

products gene name :

[NEFL]

other gene names :

[NEFL; NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; NF68; NFL; NF-L]

uniprot entry name :

NFL_HUMAN

host :

E.coli

purity :

> 97%

form :

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin 300.

concentration :

Original Concentration: 200ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

SDS-Page

image2 heading :

Sequence

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Lys281~Glu396. Tags: N-terminal His Tag

other info2 :

Predicted isoelectric point: 5.9. Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Traits: Freeze-dried powder

ncbi gi num :

105990539

ncbi acc num :

NP_006149.2

ncbi gb acc num :

NM_006158.4

uniprot acc num :

P07196

ncbi mol weight :

Predicted Molecular Mass: 14.5kDa. Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.

ncbi pathways :

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Post NMDA Receptor Activation Events Pathway (161035); Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway (161037)

ncbi summary :

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

uniprot summary :

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 8p21. Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol. Molecular Function: protein C-terminus binding; protein binding, bridging; identical protein binding; protein domain specific binding; protein binding; phospholipase binding; structural constituent of cytoskeleton. Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; axon regeneration in the peripheral nervous system; retrograde axon cargo transport; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; locomotion; neuromuscular process controlling balance; anterograde axon cargo transport; neurite morphogenesis; response to corticosterone stimulus. Disease: Charcot-marie-tooth Disease, Demyelinating, Type 1f; Charcot-marie-tooth Disease, Axonal, Type 2e

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

245

size3 :

0.1 mg

price3 :

365

size4 :

0.2 mg

price4 :

450

size5 :

0.5 mg

price5 :

870

size6 :

1 mg

price6 :

1280

more info or order :

MyBioSource product webpage