catalog number :
MBS2009755
products type :
Recombinant Protein
products full name :
Recombinant Golgi Glycoprotein 1 (GLG1)
products short name :
Golgi Glycoprotein 1 (GLG1)
other names :
Golgi apparatus protein 1 isoform 2; Golgi apparatus protein 1; Golgi apparatus protein 1; E-selectin ligand 1; golgi sialoglycoprotein MG-160; cysteine-rich fibroblast growth factor receptor; golgi glycoprotein 1; CFR-1; Cysteine-rich fibroblast growth factor receptor; E-selectin ligand 1; ESL-1; Golgi sialoglycoprotein MG-160
products gene name :
GLG1
other gene names :
GLG1; GLG1; CFR-1; ESL-1; MG160; MG-160; RP11-252A24.7; CFR1; ESL1; MG160; ESL-1
uniprot entry name :
GSLG1_HUMAN
sequence :
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-KEV LNMLKESKAD IFVDPVLHTA CALDIKHHCA AITPGRGRQM SCLMEALEDK RVRLQPECKK RLNDRIEMWS YAAKVAPADG FSDLAMQVMT SPSKN
form :
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Lys1048~Asn1145 (Accession # Q92896) with two N-terminal Tags, His-tag and T7-tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Subcellular Location: Golgi apparatus. Predicted isoelectric point: 8.8
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_001139138.1
ncbi gb acc num :
NM_001145666.1
ncbi mol weight :
14.8kDa
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); TNF-alpha/NF-kB Signaling Pathway (198884)
uniprot summary :
GLG1: Binds fibroblast growth factor and E-selectin (cell- adhesion lectin on endothelial cells mediating the binding of neutrophils). 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Vesicle. Chromosomal Location of Human Ortholog: 16q22.3. Cellular Component: Golgi membrane; Golgi apparatus; extracellular matrix; membrane; plasma membrane; integral to membrane. Molecular Function: receptor binding. Biological Process: regulation of chondrocyte differentiation; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; leukocyte migration