Recombinant Promyelocytic Leukemia Protein (PML) | MyBioSource MBS2009754 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Promyelocytic Leukemia Protein (PML)

catalog :

MBS2009754

quantity :

0.01 mg

price :

130 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2009754

products type :

Recombinant Protein

products full name :

Recombinant Promyelocytic Leukemia Protein (PML)

products short name :

[Promyelocytic Leukemia Protein (PML)]

other names :

[protein PML isoform 6; Protein PML; protein PML; RING finger protein 71; promyelocytic leukemia protein; tripartite motif protein TRIM19; probable transcription factor PML; promyelocytic leukemia, inducer of; tripartite motif-containing protein 19; promyelocytic leukemia; Promyelocytic leukemia protein; RING finger protein 71; Tripartite motif-containing protein 19]

products gene name :

[PML]

other gene names :

[PML; PML; MYL; RNF71; PP8675; TRIM19; MYL; PP8675; RNF71; TRIM19]

uniprot entry name :

PML_HUMAN

host :

E.coli

purity :

> 97%

form :

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

concentration :

Original Concentration: 200 Ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

SDS-Page

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Gln59~Glu239. Tags: N-terminal His Tag. Subcellular Location: Nucleus, Cytoplasm. Traits: Freeze-dried powder. Buffer: 10mM PBS, pH7.4, containing 5% trehalose 0.01% sarcosyl.

other info2 :

Predicted isoelectric point: 5.9. Predicted Molecular Mass: 27.2kDa. Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. USAGE: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

4505903

ncbi acc num :

NP_002666.1

ncbi gb acc num :

NM_002675.3

uniprot acc num :

P29590

ncbi pathways :

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Cytokine Signaling In Immune System Pathway (366171); DNA Damage Response Pathway (198788); Direct P53 Effectors Pathway (137939); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Immune System Pathway (106386)

ncbi summary :

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

PML: a zinc-finger protein that can regulate transcription and can localize to nuclear bodies. Cytoplasmic forms regulate glycolysis by inhibiting the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Sumoylated forms interact with SATB1 and localize to the PML nuclear bodies. Sumoylation on three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The B1 box and the RING finger are also required for localization to nuclear bodies. May play an important role in recruitment of ELF4 into PML nuclear bodies. A chromosomal aberration involving PML can cause acute promyelocytic leukemia (APL). Seven alternatively-spliced human isoforms have been reported. Protein type: Ubiquitin conjugating system; Nucleolus; Oncoprotein; Transcription factor; Tumor suppressor. Chromosomal Location of Human Ortholog: 15q22. Cellular Component: nucleoplasm; PML body; nuclear membrane; nuclear matrix; early endosome membrane; cytoplasm; nucleolus; cytosol; nucleus. Molecular Function: protein binding; protein homodimerization activity; SUMO binding; DNA binding; zinc ion binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; SMAD binding; cobalt ion binding. Biological Process: retinoic acid receptor signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; viral reproduction; apoptosis; regulation of MHC class I biosynthetic process; positive regulation of apoptosis involved in mammary gland involution; PML body organization and biogenesis; SMAD protein nuclear translocation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; entrainment of circadian clock by photoperiod; negative regulation of cell proliferation; positive regulation of histone deacetylation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; response to gamma radiation; protein complex assembly; negative regulation of mitotic cell cycle; maintenance of protein localization in nucleus; circadian regulation of gene expression; cell cycle arrest; negative regulation of telomere maintenance via telomerase; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to UV; caspase activation; regulation of protein amino acid phosphorylation; cell fate commitment; protein stabilization; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; common-partner SMAD protein phosphorylation; regulation of circadian rhythm; negative regulation of telomerase activity; endoplasmic reticulum calcium ion homeostasis; negative regulation of angiogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; response to hypoxia; innate immune response; negative regulation of translation in response to oxidative stress; myeloid cell differentiation; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; induction of apoptosis by oxidative stress; protein targeting

size1 :

0.01 mg

price1 :

130 USD

size2 :

0.05 mg

price2 :

225

size3 :

0.1 mg

price3 :

340

size4 :

0.2 mg

price4 :

415

size5 :

0.5 mg

price5 :

800

size6 :

1 mg

price6 :

1180

more info or order :

MyBioSource product webpage