protein

Recombinant Apelin (APLN)

MBS2009735

0.01 mg

135 USD

Recombinant Protein

Recombinant Apelin (APLN)

[Apelin (APLN)]

[apelin preproprotein; Apelin; apelin; AGTRL1 ligand; APJ endogenous ligand; apelin; APJ endogenous ligand]

[APLN]

[APLN; APLN; APEL; XNPEP2; APEL]

APEL_HUMAN

E. coli

>95%

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01%skl, 5% Trehalose and Proclin300.

Original Concentration: 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-PAGE

Testing Data

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Ser24~Phe77. Tags: Two N-terminal Tags, His-tag and MBP-tag. Subcellular Location: Secreted. Traits: Freeze-dried powder. Predicted isoelectric point: 6.3. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

45120090

NP_059109.3

NM_017413.4

Q9ULZ1

Predicted: 56.1kDa. Accurate: 56kDa as determined by SDS-PAGE reducing conditions

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.[provided by RefSeq, Feb 2010]

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: Xq25. Cellular Component: extracellular space; extracellular region. Molecular Function: G-protein-coupled receptor binding; hormone activity; apelin receptor binding; receptor binding. Biological Process: lactation; immune response; signal transduction

0.01 mg

135 USD

0.05 mg

245

0.1 mg

365

0.2 mg

450

0.5 mg

870

1 mg

1280