catalog number :
MBS2009721
products type :
Recombinant Protein
products full name :
Recombinant Pepsinogen A (PGA)
products short name :
Pepsinogen A (PGA)
other names :
pepsinogen 5, group I; Pepsinogen 5, group I (Pepsinogen A); pepsinogen 5, group I; pepsinogen F protein; pepsinogen 5, group I (pepsinogen A); pepsinogen 5, group I
other gene names :
Pga5; LOC100910889; Pepf; Pga5
uniprot entry name :
Q9JJX2_RAT
sequence :
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-LVKIP LMKIKSMREN LRESHMLKDY LEKYPRSRAH VLLEQRRNPS VTYEPMRNYL DLVYIGTISI GTPPQEFKVV LDTGSSDLWV PSIYCSSPAC AHHKVFNPLQ SSTFLVSGRP VNVAYGSGEM SGFLAYDTVK IGDLTVVAQA FGLSLEEPGR FMEHAVFDGI LGLGYPNLGL QGVTPVFDNL WIQGLIPQNL FAFYLSSKDE KGSVLMLGGV DPSYYHGELH WVPVSKPSYW QLAVDSISMN GEIIACDGGC QGIMDTGTSL VTGPRSSILN IQNLIGAKAS GDGEYFLKCD TINTLPDIVF TIDSVTYPVP ASAYIRKDHS HNCRSNFEES TDDPSDPELW VLGDVFLRLY FTVFDRANNR IGLASAA
form :
Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
app notes :
Accurate protein sizing on SDS-polyacrylamide gells and Western Blots
other info1 :
Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Leu16~Ala387 (Accession # Q9JJX2) with N-terminal His-Tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted Isoelectric Point: 5.6. Storage BUffer: 62.5mM Tris-H3PO4 (pH 7.5 at 25degC), 1mM EDTA, 2% (w/v) SDS, 100mM DTT, 1mM NaN3, 0.01% (w/v) bromo-phenol blue and 33% (v/v) glycerol.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_068521.1
ncbi gb acc num :
NM_021753.1
ncbi mol weight :
42.6kDa
ncbi pathways :
Protein Digestion And Absorption Pathway (172816); Protein Digestion And Absorption Pathway (171868)
ncbi summary :
neontal zymogen of pepsins, the major proteolytic enzymes in gastric juices [RGD, Feb 2006]
uniprot summary :
Sequence similarities: Belongs to the peptidase A1 family. RuleBase RU000454