protein

Recombinant Neuregulin 1 (NRG1)

MBS2009710

0.01 mg

165 USD

Recombinant Protein

Recombinant Neuregulin 1 (NRG1)

Neuregulin 1 (NRG1)

pro-neuregulin-1, membrane-bound isoform isoform 6; Pro-neuregulin-1, membrane-bound isoform; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; neuregulin 1 type III beta 3; neuregulin 1; Acetylcholine receptor-inducing activity; ARIA; Glial growth factor; Heregulin; HRG; Neu differentiation factor; Sensory and motor neuron-derived factor

NRG1

Nrg1; Nrg1; Ndf; Pro-NRG1; ARIA; HRG

NRG1_RAT

E Coli

241

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-TKKQRQKLHD RLRQSLRSER SNLVNIANGP HHPNPPPENV QLVNQYVSKN VISSEHIVER EVETSFSTSH YTSTAHHSTT VTQTPSHSWS NGHTESVISE SNSVIMMSSV ENSRHSSPAG GPRGRLHGLG

> 95%

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Thr264~Gly393 (Accession # P43322) with N-terminal His-Tag. Predicted isoelectric point: 9.3. Accurate Molecular Mass: 20 & 18kDa as determined by SDS-PAGE reducing conditions.

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

402794669

NP_001258052.1

NM_001271123.1

P43322

15.9kDa

Adaptive Immune System Pathway (936651); Constitutive PI3K/AKT Signaling In Cancer Pathway (936222); DAP12 Interactions Pathway (936753); DAP12 Signaling Pathway (936754); Disease Pathway (936186); Downregulation Of ERBB2:ERBB3 Signaling Pathway (936078); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (936665); Downstream Signal Transduction Pathway (936065); Downstream Signaling Of Activated FGFR Pathway (935996); ErbB Signaling Pathway (83441)

ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006]

NRG1: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. The cytoplasmic domain interacts with the LIM domain region of LIMK1. Interacts with ERBB3 and ERBB4. Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. Belongs to the neuregulin family. 10 isoforms of the human protein are produced by alternative splicing. Protein type: Ligand, receptor tyrosine kinase; Cell development/differentiation; Membrane protein, integral; Cytokine; Motility/polarity/chemotaxis. Cellular Component: extracellular space; integral to plasma membrane; axon; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; synapse; neuromuscular junction. Molecular Function: ErbB-2 class receptor binding; ErbB-3 class receptor binding; growth factor activity; transcription cofactor activity; protein tyrosine kinase activator activity; receptor binding. Biological Process: myelination; regulation of protein heterodimerization activity; muscle development; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of cell adhesion; wound healing; cellular protein complex disassembly; heart development; ventricular cardiac muscle cell differentiation; cell morphogenesis; locomotory behavior; glucose transport; synaptogenesis; glial cell differentiation; regulation of blood pressure; regulation of cell differentiation; organ development; cell differentiation; positive regulation of cardiac muscle cell proliferation; nervous system development; cell migration; neurotransmitter receptor metabolic process; MAPKKK cascade; neuron fate commitment; positive regulation of cell growth; peripheral nervous system development; cell proliferation; positive regulation of protein kinase B signaling cascade; glial cell fate commitment; positive regulation of protein kinase activity; negative regulation of secretion; positive regulation of Ras protein signal transduction; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway

0.01 mg

165 USD

0.05 mg

325

0.1 mg

505