Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12) | MyBioSource MBS2009620 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12)

catalog :

MBS2009620

quantity :

0.01 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2009620

products type :

Recombinant Protein

products full name :

Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12)

products short name :

17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12)

other names :

estradiol 17-beta-dehydrogenase 12; Estradiol 17-beta-dehydrogenase 12; estradiol 17-beta-dehydrogenase 12; KAR; 17-beta-HSD 12; 3-ketoacyl-CoA reductase; 17-beta-hydroxysteroid dehydrogenase 12; smooth muscle-specific 17 beta-hydroxysteroid dehydrogenase type 3; hydroxysteroid (17-beta) dehydrogenase 12; 17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase

products gene name :

HSD17b12

other gene names :

Hsd17b12; Hsd17b12; 17-beta-HSD 12; KAR

uniprot entry name :

DHB12_RAT

host :

E Coli

sequence length :

291

sequence :

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-LYWVGASTIA YLTLRASYSL FRAFQVWCVG NQAFVGPRLG EWAVVTGGTD GIGKSYAEEL AKRGMKIVLI SRSQDKLKEV SNNIKEKFNV ETRTIAVDFS LDDIYDKIKT GLSGLEIGVL VNNVGMSYEY PEYFLEIPDL DNTIKKLINI NVLSICKVTR LVLPGMVERS KGVILNISSA SGMLPVPLLT VYSATKAFVD FFSQCLHEEY KSKGIFVQSV LPFFVATKLA KIRKPTLDKP SAETFVKSAI KTVGLQTRTT GYVIHAIMGS INSILPRWIY F

purity :

> 95%

form :

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Leu11~Phe291 (Accession # Q6P7R8) with two N-terminal Tags, His-tag and GST-tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

14091750

ncbi acc num :

NP_114455.1

ncbi gb acc num :

NM_032066.1

uniprot acc num :

Q6P7R8

ncbi mol weight :

58.3kDa

ncbi pathways :

Biosynthesis Of Unsaturated Fatty Acids Pathway (83426); Biosynthesis Of Unsaturated Fatty Acids Pathway (429); Fatty Acyl-CoA Biosynthesis Pathway (935491); Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway (434796); Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway (468402); Fatty Acid Elongation Pathway (83327); Fatty Acid Elongation Pathway (295); Fatty Acid Metabolism Pathway (869319); Fatty Acid Metabolism Pathway (878045); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (935489)

ncbi summary :

may play a role in steroid metabolism [RGD, Feb 2006]

uniprot summary :

HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily. Protein type: Membrane protein, integral; Lipid Metabolism - unsaturated fatty acid biosynthesis; Lipid Metabolism - androgen and estrogen; Oxidoreductase; Membrane protein, multi-pass; EC 1.1.1.62. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; endoplasmic reticulum membrane; integral to membrane. Molecular Function: collagen binding; estradiol 17-beta-dehydrogenase activity; heparin binding; fibronectin binding; oxidoreductase activity. Biological Process: extracellular matrix organization and biogenesis; estrogen biosynthetic process; fatty acid biosynthetic process

size1 :

0.01 mg

price1 :

200 USD

size2 :

0.05 mg

price2 :

430

size3 :

0.1 mg

price3 :

665

more info or order :

MyBioSource product webpage