protein

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

MBS2009607

0.01 mg

250 USD

Recombinant Protein

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

A disintegrin and metalloproteinase with thrombospondin motifs 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; ADAM-TS1; ADAMTS-1; ADAM-TS 1; human metalloproteinase with thrombospondin type 1 motifs; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; METH-1

ADAMTS1

ADAMTS1; ADAMTS1; C3-C5; METH1; KIAA1346; METH1; ADAM-TS 1; ADAM-TS1; ADAMTS-1

ATS1_HUMAN

E Coli

967

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGS-TFSAWVI EEWGECSKSC ELGWQRRLVE CRDINGQPAS ECAKEVKPAS TRPCADHPCP QWQLGEWSSC SKTCGKGYKK RSLKCLSHDG GVLSHESCDP LKKPKHFIDF CTMAECS

> 95%

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Thr854~Ser967 (Accession # Q9UHI8) with N-terminal His-Tag

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

50845384

NP_008919.3

NM_006988.3

Q9UHI8

14.0kDa

Degradation Of The Extracellular Matrix Pathway 576263!!Degradation Of The Extracellular Matrix Pathway 934113!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!Extracellular Matrix Organization Pathway 934100

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover . By similarity. Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. Ref.2. Catalytic activity: Cleaves aggrecan at the 1938-Glu- -Leu-1939 site, within the chondroitin sulfate attachment domain. Cofactor: Binds 1 zinc ion per subunit. Ref.8. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase . By similarity.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 3 TSP type-1 domains. Sequence caution: The sequence AAD48080.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA92584.1 differs from that shown. Reason: Erroneous initiation.

0.01 mg

250 USD