Recombinant Thioredoxin (Trx) | MyBioSource MBS2009585 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Thioredoxin (Trx)

catalog :

MBS2009585

quantity :

0.01 mg

price :

185 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2009585

products type :

Recombinant Protein

products full name :

Recombinant Thioredoxin (Trx)

products short name :

Thioredoxin (Trx)

other names :

thioredoxin; Thioredoxin; thioredoxin; trx; thioredoxin 1

products gene name :

Trx

other gene names :

Txn1; Txn; Txn; Txn1; Trx

uniprot entry name :

THIO_RAT

host :

E Coli

sequence length :

105

sequence :

The target protein is fused with a N-terminal his-tag, its sequence is listed below. MGHHHHHHSGSEF-MVKLIESKEA FQEALAAAGD KLVVVDFSAT WCGPCKMIKP FFHSLCDKYS NVVFLEVDVD DCQDVAADCE VKCMPTFQFY KKGQKVGEFS GANKEKLEAT ITEFA

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: MET1~Ala105 (Accession # P11232) with a N-terminal his-tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted isoelectric point: 5.6. Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

16758644

ncbi acc num :

NP_446252.1

ncbi gb acc num :

NM_053800.3

uniprot acc num :

P11232

ncbi mol weight :

13.2kDa

ncbi pathways :

Cellular Senescence Pathway (935708); Cellular Responses To Stress Pathway (935701); Detoxification Of Reactive Oxygen Species Pathway (935707); Immune System Pathway (936650); Inflammasomes Pathway (936724); Innate Immune System Pathway (936679); Metabolism Pathway (935443); Metabolism Of Nucleotides Pathway (935606); NFE2L2 Pathway (739005); Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways (936722)

ncbi summary :

protein-disulfide reductase enzyme that is important for redox signalling during apoptosis and cell growth [RGD, Feb 2006]

uniprot summary :

TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family. Protein type: Nuclear receptor co-regulator. Cellular Component: cell soma; mitochondrion; axon; dendrite; cytoplasm; extracellular region; cytosol; nucleus. Molecular Function: thioredoxin-disulfide reductase activity; enzyme binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity. Biological Process: response to drug; negative regulation of protein export from nucleus; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; response to selenium ion; response to radiation; regulation of transcription, DNA-dependent; cell redox homeostasis; regulation of protein import into nucleus, translocation; response to activity; response to axon injury; positive regulation of DNA binding

size1 :

0.01 mg

price1 :

185 USD

size2 :

0.05 mg

price2 :

380

size3 :

0.1 mg

price3 :

585

more info or order :

MyBioSource product webpage