catalog number :
MBS2009521
products type :
Recombinant Protein
products full name :
Recombinant Artemin (ARTN)
products short name :
Artemin (ARTN)
other names :
artemin isoform 1; Artemin; artemin; artemin
products gene name :
ARTN
other gene names :
Artn; Artn; neublastin
uniprot entry name :
ARTN_MOUSE
sequence :
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-AGTRSSRAR TTDARGCRLR SQLVPVSALG LGHSSDELIR FRFCSGSCRR ARSQHDLSLA SLLGAGALRS PPGSRPISQP CCRPTRYEAV SFMDVNSTWR TVDHLSATAC GCLG
form :
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Ala112~Gly224 (Accession # Q9Z0L2) with N-terminal His-Tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_001271120.1
ncbi gb acc num :
NM_001284191.1
ncbi mol weight :
13.6kDa
ncbi pathways :
Axon Guidance Pathway (926383); Developmental Biology Pathway (926382); NCAM Signaling For Neurite Out-growth Pathway (926392); NCAM1 Interactions Pathway (926393)
uniprot summary :
artemin: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer s patch-like structures, a major component of the gut-associated lymphoid tissue. Belongs to the TGF-beta family. GDNF subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Molecular Function: glial cell line-derived neurotrophic factor receptor binding; protein binding; growth factor activity; receptor binding. Biological Process: axon guidance; cell surface receptor linked signal transduction; peripheral nervous system development; induction of positive chemotaxis