protein

Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1)

MBS2009504

0.01 mg

195 USD

Recombinant Protein

Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1)

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1)

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; tiptoe walking; lymphocyte antigen 41; plasma-cell membrane glycoprotein PC-1; phosphodiesterase I/nucleotide pyrophosphatase 1; 1 ectonucleotide pyrophosphatase/phosphodiesterase 1; ectonucleotide pyrophosphatase/phosphodiesterase 1; Lymphocyte antigen 41; Ly-41; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1

ENPP1

Enpp1; Enpp1; Pca; ttw; twy; M6S1; NPP1; Npps; PC-1; Ly-41; Pca-1; Pdnp1; C76301; CD203c; E-NPP1; AI428932; 4833416E15Rik; Npps; Pc1; Pdnp1; E-NPP 1; Ly-41; NPPase

ENPP1_MOUSE

E Coli

906

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-GSH GSLNHLLKKP IYNPSHPKEE GFLSQCPIKS TSNDLGCTCD PWIVPIKDFE KQLNLTTEDV DDIYHMTVPY GRPRILLKQH HVCLLQQQQF LTGYSLDLLM PLWASYTFLR NDQFSRDDFS NCLYQDLRIP LSPVHKCSYY KSNSKLSYGF LTPPRLNRVS NHIYSEALLT SNIVPMYQSF QVIWHYLHDT LLQRYAHERN GINVVSGPVF DFDYDGRYD

> 95%

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH 8.0, containing 1mM EDTA, 1mMDTT, 5% trehalose, 0.01% sarcosyl.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Gly568~Asp789 (Accession # P06802) with two N-terminal Tags, His-tag and GST-tag. Subcellular Location: Cell membrane; Secreted . Single-pass type II membrane protein. Basolateral cell membrane. Predicted isoelectric point: 6.4

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

23503085

P06802.4

P06802

558.3kDa

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (926334); Defective BTD Causes Biotidinase Deficiency Pathway (926362); Defective CD320 Causes Methylmalonic Aciduria Pathway (926359); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (926348); Defective GIF Causes Intrinsic Factor Deficiency Pathway (926351); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (926361); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (926350); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (926357); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (926356); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (926352)

This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5 triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3 ,5 -cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Protein type: EC 3.6.1.9; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - riboflavin; Phosphodiesterase; Motility/polarity/chemotaxis; Membrane protein, integral; Nucleotide Metabolism - purine; EC 3.1.4.1; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Carbohydrate Metabolism - starch and sucrose. Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; lysosomal membrane; integral to membrane; plasma membrane; extracellular region. Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; protein homodimerization activity; zinc ion binding; hydrolase activity; metal ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; insulin receptor binding; polysaccharide binding; nucleic acid binding; catalytic activity; ATP binding; scavenger receptor activity. Biological Process: generation of precursor metabolites and energy; sequestering of triacylglycerol; metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; bone remodeling; negative regulation of fat cell differentiation; phosphate metabolic process; 3 -phosphoadenosine 5 -phosphosulfate metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process

0.01 mg

195 USD

0.05 mg

425

0.1 mg

660