protein

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

MBS2009500

0.01 mg

255 USD

Recombinant Protein

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADMP-1; Aggrecanase-1

ADAMTS4

ADAMTS4; ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4; KIAA0688; ADAM-TS 4; ADAM-TS4; ADAMTS-4

ATS4_HUMAN

E Coli

837

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-TFPG KDYDADRQCQ LTFGPDSRHC PQLPPPCAAL WCSGHLNGHA MCQTKHSPWA DGTPCGPAQA CMGGRCLHMD QLQDFNIPQA GGWGPWGPWG DCSRTCGGGV QFSSRDCTRP VPRNGGKYCE GRRTRFRSCN TEDCP

> 95%

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Thr437~Pro575 (Accession # O75173) with N-terminal His-Tag

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

157427675

NP_005090.3

NM_005099.4

O75173

16.7kDa

Degradation Of The Extracellular Matrix Pathway 576263!!Degradation Of The Extracellular Matrix Pathway 934113!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!Extracellular Matrix Organization Pathway 934100

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu- -Ala-393' site. Catalytic activity: Glutamyl endopeptidase; bonds cleaved include 370-Thr-Glu-Gly-Glu- -Ala-Arg-Gly-Ser-377 in the interglobular domain of mammalian aggrecan. Cofactor: Binds 1 zinc ion per subunit. Ref.9. Subunit structure: Interacts with SRPX2. Ref.8. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Tissue specificity: Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Induction: By IL1/interleukin-1. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 TSP type-1 domain. Caution: Has sometimes been referred to as ADAMTS2. Sequence caution: The sequence BAA31663.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.01 mg

255 USD