Recombinant Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) | MyBioSource MBS2009445 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)

catalog :

MBS2009445

quantity :

0.01 mg

price :

195 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2009445

products type :

Recombinant Protein

products full name :

Recombinant Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)

products short name :

Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)

other names :

serine protease inhibitor Kazal-type 5 isoform a; Serine protease inhibitor Kazal-type 5; serine protease inhibitor Kazal-type 5; serine protease inhibitor, Kazal type 5; lymphoepithelial Kazal-type-related inhibitor; lympho-epithelial Kazal-type-related inhibitor; serine peptidase inhibitor, Kazal type 5; Lympho-epithelial Kazal-type-related inhibitor

products gene name :

SPINK5

other gene names :

SPINK5; SPINK5; NS; NETS; LEKTI; LETKI; VAKTI; LEKTI

uniprot entry name :

ISK5_HUMAN

host :

E Coli

reactivity :

Homo sapiens (Human)

sequence length :

1094

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GG GNTQDECAEY REQMKNGRLS CTRESDPVRD ADGKSYNNQC TMCKAKLERE AERKNEYSRS RSNGTGSESG KDTCDEFRSQ MKNGKLICTR ESDPVRGPDG KTHGNKCTMC KEKLEREAAE KKKKEDEDRS NTGERSNTGE RSNDKEDLCR EFRSMQRNGK LICTRENNPV RGPYGKMHIN KCAMCQSIFD REANERKKKD EEKSSSKPSN NAKDECSEFR NYIRNNELIC PRENDPVHGA DGKFYTNKCY MCRAVFLTEA LERAKLQEKP SHVRAS

purity :

> 95%

form :

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Expression System: Prokaryotic expression. Residues: Gly699~Ser976 (Accession # Q9NQ38) with two N-terminal Tags, His-tag and T7-tag. Predicted isoelectric point: 8.8

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

189163501

ncbi acc num :

NP_001121170.1

ncbi gb acc num :

NM_001127698.1

uniprot acc num :

Q9NQ38

ncbi mol weight :

35.7kDa

ncbi summary :

This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The inhibitor may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SPINK5: Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense- activating and desquamation-involved proteases. Inhibits KLK5, it s major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. Defects in SPINK5 are the cause of Netherton syndrome (NETH). NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Inhibitor. Chromosomal Location of Human Ortholog: 5q32. Cellular Component: endoplasmic reticulum membrane; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; extracellular region; cell cortex; cytosol. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; epithelial cell differentiation; negative regulation of immune response; hair cell differentiation; epidermal cell differentiation; regulation of T cell differentiation; anagen. Disease: Ige Responsiveness, Atopic; Netherton Syndrome

size1 :

0.01 mg

price1 :

195 USD

size2 :

0.05 mg

price2 :

425

size3 :

0.1 mg

price3 :

660

more info or order :

MyBioSource product webpage