protein

Recombinant Heparanase (HPA)

MBS2009412

0.01 mg

175 USD

Recombinant Protein

Recombinant Heparanase (HPA)

Heparanase (HPA)

heparanase isoform 1 preproprotein; Heparanase; heparanase; heparanase-1; endo-glucoronidase; heparanase; Endo-glucoronidase; Heparanase-1

HPA

HPSE; HPSE; HPA; HPA1; HPR1; HSE1; HPSE1; HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Hpa1

HPSE_HUMAN

E Coli

543

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-PLPDYWLSLL FKKLVGTKVL MASVQGSKRR KLRVYLHCTN TDNPRYKEGD LTLYAINLHN VTKYLRLPYP FSNKQVDKYL LRPLGPHGLL SKSVQLNGLT LKMVDDQTLP PLMEKPLRPG SSLGLPAFSY SFFVIRNAKV AACI

> 95%

Supplied as lyophilized form in PBS, pH 7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Pro400~Ile543 (Accession # Q9Y251) with N-terminal His-Tag. Predicted Isoelectric Point: 9.9. Predicted Molecular Mass: 17.9kDa

Subcellular Location: Lysosome membrane. Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

148746204

NP_001092010.1

NM_001098540.2

Q9Y251

17.9kDa

Disease Pathway (530764); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355); Glycosaminoglycan Metabolism Pathway (645297); HS-GAG Degradation Pathway (645307); Heparan Sulfate Degradation Pathway (413377); Heparan Sulfate Degradation Pathway (468270); Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway (645304); MPS I - Hurler Syndrome Pathway (685537); MPS II - Hunter Syndrome Pathway (685538)

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family. Protein type: Glycan Metabolism - glycosaminoglycan degradation; Secreted, signal peptide; Hydrolase; EC 3.2.1.166; Extracellular matrix; Secreted. Chromosomal Location of Human Ortholog: 4q21.3. Cellular Component: nucleoplasm; lysosomal lumen; intracellular membrane-bound organelle; lysosome; lysosomal membrane; extracellular region; nucleus; lipid raft. Molecular Function: syndecan binding; protein dimerization activity; protein binding; beta-glucuronidase activity; heparanase activity. Biological Process: positive regulation of hair follicle development; proteoglycan metabolic process; positive regulation of protein kinase B signaling cascade; glycosaminoglycan catabolic process; positive regulation of blood coagulation; heparan sulfate proteoglycan catabolic process; glycosaminoglycan metabolic process; cell-matrix adhesion; positive regulation of osteoblast proliferation; carbohydrate metabolic process; pathogenesis; regulation of hair follicle development

0.01 mg

175 USD

0.05 mg

375

0.1 mg

580