catalog number :
MBS2009404
products type :
Recombinant Protein
products full name :
Recombinant Artemin (ARTN)
products short name :
Artemin (ARTN)
other names :
artemin; Artemin; artemin; artemin
products gene name :
ARTN
other gene names :
Artn; Artn
uniprot entry name :
ARTN_RAT
sequence :
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-AGTRSSRAR ATDARGCRLR SQLVPVSALG LGHSSDELIR FRFCSGSCRR ARSPHDLSLA SLLDAGALRS PPGSRPISQP CCRPTRYEAV SFMDVNSTWR TVDHLSATAC GCLG
form :
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Ala112~Gly224 (Accession # Q6AYE8) with two N-terminal Tags, His-tag and T7-tag. Predicted Isoelectric point: 10.5. Subcellular Location: Secreted
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_445849.1
ncbi gb acc num :
NM_053397.1
ncbi mol weight :
15.9kDa
uniprot summary :
Function: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue . By similarity. Subunit structure: Homodimer; disulfide-linked. Binds to RET . By similarity. Subcellular location: Secreted . By similarity. Tissue specificity: Cochlea. Expressed at higher level in sesorineural epithelium than in the modiolus region or substantia nigra. Ref.3 Ref.4. Developmental stage: Expressed during embryogenesis. At embryonic day 14 (E14) not detected in the brain or spinal cord. A striking expression seen in the nerve roots but not in the developing neurons of the dorsal root ganglia. A significant expression also seen around the superior mesentric artery. Ref.4. Sequence similarities: Belongs to the TGF-beta family. GDNF subfamily.