protein

Recombinant Interleukin 23 (IL23)

MBS2009384

0.01 mg

205 USD

Recombinant Protein

Recombinant Interleukin 23 (IL23)

Interleukin 23 (IL23)

interleukin-23 subunit alpha; Interleukin-23 subunit alpha; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor; interleukin 23, alpha subunit p19; Interleukin-23 subunit p19

IL23

IL23A; IL23A; P19; SGRF; IL-23; IL-23A; IL23P19; SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19

IL23A_HUMAN

E Coli

189

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF- R AVPGGSSPAW TQCQQLSQKL CTLAWSAHPL VGHMDLREEG DEETTNDVPH IQCGDGCDPQ GLRDNSQFCL QRIHQGLIFY EKLLGSDIFT GEPSLLPDSP VGQLHASLLG LSQLLQPEGH HWETQQIPSL SPSQPWQRLL LRFKILRSLQ AFVAVAARVF AHGAATLSP

> 95%

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Arg20~Pro189 (Accession # Q9NPF7) with N-terminal His-Tag. Subcellular Location: Secreted

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted Isoelectric Point: 6.1. Accurate Molecular Mass: 15 & 16kDa as determined by SDS-PAGE reducing conditions.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

7706702

NP_057668.1

NM_016584.2

Q9NPF7

20.2kDa

ATF-2 Transcription Factor Network Pathway (138006); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL23-mediated Signaling Events Pathway (138000); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Pertussis Pathway (218111); Pertussis Pathway (218099)

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008]

IL23A: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Belongs to the IL-6 superfamily. Protein type: Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 12q13.3. Molecular Function: protein binding; interleukin-23 receptor binding; cytokine activity. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of interleukin-10 production; positive regulation of T-helper 1 type immune response; positive regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; positive regulation of osteoclast differentiation; tissue remodeling; positive regulation of NK T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; positive regulation of NF-kappaB import into nucleus; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; positive regulation of natural killer cell activation; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; T cell proliferation; positive regulation of T cell proliferation; inflammatory response; defense response to virus; positive regulation of memory T cell differentiation; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of NK T cell activation; positive regulation of tumor necrosis factor production; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; positive regulation of inflammatory response

0.01 mg

205 USD

0.05 mg

460

0.1 mg

715