Recombinant Apolipoprotein B (APOB) | MyBioSource MBS2009355 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Apolipoprotein B (APOB)

catalog :

MBS2009355

quantity :

0.01 mg

price :

105 USD

more info or order :

MyBioSource product webpage

image

image 1 :

Figure. SDS-PAGE

image 2 :

Figure. Gene Sequencing (Extract)

product information

catalog number :

MBS2009355

products type :

Recombinant Protein

products full name :

Recombinant Apolipoprotein B (APOB)

products short name :

[Apolipoprotein B (APOB)]

other names :

[apolipoprotein B-100; Apolipoprotein B-100; apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen); apolipoprotein B]

products gene name :

[APOB]

other gene names :

[APOB; APOB; FLDB; LDLCQ4; Apo B-100; Apo B-48]

uniprot entry name :

APOB_HUMAN

host :

E. coli

sequence :

HLLSSS SSVIDALQYK LEGTTRLTRK RGLKLATALS LSNKFVEGSH NSTVSLTTKN MEVSVATTTK AQIPILRMNF KQELNGNTKS KPTVSSSMEF KYDFNSSMLY STAKGAVDHK LSLESLTSYF SIESSTKGDV KGSVLSREYS GTIASEANTY LNSKSTRSSV KLQGTSKIDD IWNLEVKE

purity :

> 90%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

concentration :

Original Concentration: 200 ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)

app notes :

(May be suitable for use in other assays to be determined by the end user.)

image1 heading :

SDS-Page

image2 heading :

Sequence

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: His3365~Glu3548. Tags: N-terminal His Tag. Subcellular Location: Secreted, Cytoplasm. Traits: Freeze-dried powder. Predicted isoelectric point: 9.5. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

105990532

ncbi acc num :

NP_000375.2

ncbi gb acc num :

NM_000384.2

uniprot acc num :

P04114

ncbi mol weight :

Predicted: 21.4 kDa. Accurate: 26 kDa as determined by SDS-PAGE reducing conditions.

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA1 Transcription Factor Network Pathway (137979); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (106028); LDL-mediated Lipid Transport Pathway (106159)

ncbi summary :

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

uniprot summary :

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. Protein type: Secreted; Secreted, signal peptide; Carrier. Chromosomal Location of Human Ortholog: 2p24-p23. Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; chylomicron; cell soma; intracellular membrane-bound organelle; endoplasmic reticulum lumen; cytoplasm; early endosome; extracellular region; plasma membrane; endosome membrane; cytosol; actin cytoskeleton. Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding. Biological Process: lipoprotein catabolic process; phototransduction, visible light; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; nervous system development; receptor-mediated endocytosis; cholesterol metabolic process; cholesterol transport; in utero embryonic development; response to virus; regulation of cholesterol biosynthetic process; lipoprotein metabolic process; cholesterol efflux; cholesterol homeostasis; sperm motility; fertilization; lipoprotein biosynthetic process; lipoprotein transport; artery morphogenesis; spermatogenesis; triacylglycerol mobilization; blood coagulation; leukocyte migration. Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.05 mg

price2 :

180

size3 :

0.1 mg

price3 :

250

size4 :

0.2 mg

price4 :

305

size5 :

0.5 mg

price5 :

580

size6 :

1 mg

price6 :

855

more info or order :

MyBioSource product webpage