Recombinant Fibulin 3 (FBLN3) | MyBioSource MBS2009345 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Fibulin 3 (FBLN3)

catalog :

MBS2009345

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2009345

products type :

Recombinant Protein

products full name :

Recombinant Fibulin 3 (FBLN3)

products short name :

Fibulin 3 (FBLN3)

other names :

EGF-containing fibulin-like extracellular matrix protein 1; EGF-containing fibulin-like extracellular matrix protein 1; EGF-containing fibulin-like extracellular matrix protein 1; fibulin-3; extracellular protein S1-5; EGF containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5; Fibrillin-like protein; Fibulin-3

products gene name :

FBLN3

other gene names :

EFEMP1; EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; FBLN3; FBNL; FIBL-3

uniprot entry name :

FBLN3_HUMAN

host :

E Coli

reactivity :

Homo sapiens (Human)

sequence length :

493

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-CDA SNQCAQQCYN ILGSFICQCN QGYELSSDRL NCEDIDECRT SSYLCQYQCV NEPGKFSCMC PQGYQVVRSR TCQDINECET TNECREDEMC WNYHGGFRCY PRNPCQDPYI LTPENRCVCP VSNAMCRELP QSIVYKYMSI RSDRSVPSDI FQIQATTIYA NTINTFRIKS GNENGEFYLR QTSPVSAMLV LVKSLSGPRE HIVDLEMLTV SSIGTFRTSS VLRLTIIVGP FSF

purity :

> 95%

form :

Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Expression System: Prokaryotic expression. Residues: Cys258~Phe493 (Accession # Q12805) with N-terminal His-Tag. Subcellular Location: Secreted, extracellular space, extracellular matrix.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted isoelectric point: 5.7. Predicted Molecular Mass: 28.4kDa. Reconstitution: Reconstitute in sterile ddH2O.

ncbi gi num :

86788015

ncbi acc num :

NP_001034437.1

ncbi gb acc num :

NM_001039348.2

uniprot acc num :

Q12805

ncbi pathways :

Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Molecules Associated With Elastic Fibres Pathway (730311)

ncbi summary :

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

uniprot summary :

EFEMP1: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD); also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2p16. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: protein binding; growth factor activity; epidermal growth factor receptor activity; calcium ion binding; epidermal growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; visual perception; negative regulation of chondrocyte differentiation. Disease: Doyne Honeycomb Retinal Dystrophy

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

395

size3 :

0.1 mg

price3 :

610

more info or order :

MyBioSource product webpage