Recombinant Defensin Alpha 1, Neutrophil (DEFa1) | MyBioSource MBS2009331 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Defensin Alpha 1, Neutrophil (DEFa1)

catalog :

MBS2009331

quantity :

0.01 mg

price :

155 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

image 3 :

product information

catalog number :

MBS2009331

products type :

Recombinant Protein

products full name :

Recombinant Defensin Alpha 1, Neutrophil (DEFa1)

products short name :

[Defensin Alpha 1, Neutrophil (DEFa1)]

other names :

[neutrophil defensin 1; Neutrophil defensin 1; neutrophil defensin 1; defensin, alpha 1B; Defensin, alpha 1; HNP-1; HP-1; HP1Cleaved into the following 2 chains:HP 1-56Neutrophil defensin 2; Alternative name(s):; HNP-2]

products gene name :

[DEFa1]

other gene names :

[DEFA1B; DEFA1; HP1; HP-1; HNP-1; DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2]

uniprot entry name :

DEF1_HUMAN

host :

E Coli

purity :

> 90%

concentration :

Original Concentration: 200 ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

image3 heading :

Testing Data

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Glu20~Cys94. Tags: N-terminal His Tag. Subcellular Location: Secreted. Traits: Freeze-dried powder. Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose

other info2 :

Predicted isoelectric point: 6.4. Predicted Molecular Mass: 9.5kDa. Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions . Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. USAGE: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

124248516

ncbi acc num :

NP_001035965.1

ncbi gb acc num :

NM_001042500.1

uniprot acc num :

P59665

ncbi pathways :

Alpha-defensins Pathway (530758); Cellular Roles Of Anthrax Toxin Pathway (138076); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

uniprot summary :

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region. Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus

size1 :

0.01 mg

price1 :

155 USD

size2 :

0.05 mg

price2 :

305

size3 :

0.1 mg

price3 :

470

size4 :

0.2 mg

price4 :

580

size5 :

0.5 mg

price5 :

1125

size6 :

1 mg

price6 :

1680

more info or order :

MyBioSource product webpage