Recombinant Collagen Type IV Alpha 5 (COL4a5) | MyBioSource MBS2009288 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Collagen Type IV Alpha 5 (COL4a5)

catalog :

MBS2009288

quantity :

0.01 mg

price :

130 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2009288

products type :

Recombinant Protein

products full name :

Recombinant Collagen Type IV Alpha 5 (COL4a5)

products short name :

Collagen Type IV Alpha 5 (COL4a5)

other names :

collagen alpha-5(IV) chain isoform 1; Collagen alpha-5(IV) chain; collagen alpha-5(IV) chain; collagen alpha-5(IV) chain; dA24A23.1; dA149D17.3; collagen IV, alpha-5 polypeptide; collagen of basement membrane, alpha-5 chain; collagen, type IV, alpha 5

products gene name :

COL4a5

other gene names :

COL4A5; COL4A5; ATS; ASLN; CA54

uniprot entry name :

CO4A5_HUMAN

host :

E Coli

sequence length :

1685

sequence :

TDAPQCPQGT LQVYEGFSLL YVQGNKRAHG QDLGTAGSCL RRFSTMPFMF CNINNVCNFA SRNDYSYWLS TPEPMPMSMQ PLKGQSIQPF ISRCAVCEAP AVVIAVHSQT IQIPHCPQGW DSLWIGYSFM MHTSAGAEGS GQALASPGSC LEEFRSAPFI ECHGRGTCNY YANSYSFWLA TVDVSDMFSK PQSETLKAGD LRTRISRCQV CMKRT
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTD
DDDKAMADIGSEF-GFLITRHSQT
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.

purity :

> 95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Gly1461~Thr1685 (Accession # P29400) with two N-terminal Tags, His-tag and S-tag. Predicted isoelectric point: 6.5. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

ncbi gi num :

4502955

ncbi acc num :

NP_000486.1

ncbi gb acc num :

NM_000495.4

uniprot acc num :

P29400

ncbi mol weight :

30.7kDa

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Axon Guidance Pathway (105688); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Developmental Biology Pathway (477129); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

uniprot summary :

COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Extracellular matrix; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: Xq22. Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina; neuromuscular junction. Molecular Function: extracellular matrix structural constituent. Biological Process: extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; neuromuscular junction development. Disease: Alport Syndrome, X-linked

size1 :

0.01 mg

price1 :

130 USD

size2 :

0.05 mg

price2 :

230

size3 :

0.1 mg

price3 :

345

more info or order :

MyBioSource product webpage