Recombinant Myeloperoxidase (MPO) | MyBioSource MBS2009287 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Myeloperoxidase (MPO)

catalog :

MBS2009287

quantity :

0.01 mg

price :

115 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2009287

products type :

Recombinant Protein

products full name :

Recombinant Myeloperoxidase (MPO)

products short name :

[Myeloperoxidase (MPO)]

other names :

[myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase]

products gene name :

[MPO]

other gene names :

[MPO; MPO; MPO]

uniprot entry name :

PERM_HUMAN

host :

E Coli

sequence length :

745

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-VTCPEQ DKYRTITGMC NNRRSPTLGA SNRAFVRWLP AEYEDGFSLP YGWTPGVKRN GFPVALARAV SNEIVRFPTD QLTPDQERSL MFMQWGQLLD HDLDFTPEPA ARASFVTG

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Val165~Gly278 (Accession # P05164) with N-terminal His-Tag. Predicted isoelectric point: 6.4. Predicted Molecular Mass: 14.4kDa

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in ddH2O.

ncbi gi num :

4557759

ncbi acc num :

NP_000241.1

ncbi gb acc num :

NM_000250.1

uniprot acc num :

P05164

ncbi mol weight :

14.4kDa

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Folate Metabolism Pathway (198833); IL23-mediated Signaling Events Pathway (138000); Phagosome Pathway (153910); Phagosome Pathway (153859); Selenium Pathway (198825); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Vitamin B12 Metabolism Pathway (920974); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

uniprot summary :

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.11.2.2. Chromosomal Location of Human Ortholog: 17q23.1. Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule. Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding. Biological Process: response to food; respiratory burst during defense response; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; response to yeast; defense response; response to lipopolysaccharide; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging. Disease: Myeloperoxidase Deficiency; Alzheimer Disease

size1 :

0.01 mg

price1 :

115 USD

size2 :

0.05 mg

price2 :

200

size3 :

0.1 mg

price3 :

290

size4 :

0.2 mg

price4 :

360

size5 :

0.5 mg

price5 :

690

size6 :

1 mg

price6 :

1015

more info or order :

MyBioSource product webpage