Recombinant Nuclear Factor Kappa B2 (NFkB2) | MyBioSource MBS2009153 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Nuclear Factor Kappa B2 (NFkB2)

catalog :

MBS2009153

quantity :

0.01 mg

price :

160 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2009153

products type :

Recombinant Protein

products full name :

Recombinant Nuclear Factor Kappa B2 (NFkB2)

products short name :

Nuclear Factor Kappa B2 (NFkB2)

other names :

nuclear factor NF-kappa-B p100 subunit isoform a; Nuclear factor NF-kappa-B p100 subunit; nuclear factor NF-kappa-B p100 subunit; oncogene Lyt-10; NFKB, p52/p100 subunit; DNA-binding factor KBF2; transcription factor NFKB2; nuclear factor Kappa-B, subunit 2; lymphocyte translocation chromosome 10 protein; nuclear factor of Kappa light chain gene enhancer in B cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10

products gene name :

NFkB2

other gene names :

NFKB2; NFKB2; p52; p105; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; LYT10; Lyt10

uniprot entry name :

NFKB2_HUMAN

host :

E Coli

sequence length :

900

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-PYL VIVEQPKQRG FRFRYGCEGP SHGGLPGASS EKGRKTYPTV KICNYEGPAK IEVDLVTHSD PPRAHAHSLV GKQCSELGIC AVSVGPKDMT AQFNNLGVLH VTKKNMMGTM IQKLQRQRLR SRPQGLTEAE QRELEQEAKE LKKVMDLSIV RLRFSAFLRA SDGSFSLPLK PVISQPIHDS KSPGASNLKI SRMDKTAGSV RGGDEVYLLC DKVQKDDIEV RFYEDDENGW QAFGDFSPTD VHKQYAIVFR TPPYHKMKIE RPVTVFLQLK RKRGGDVSDS KQFTYYPLVE DKEEVQRKRR KAL

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Pro38~Leu343 (Accession # Q00653) with two N-terminal Tags, His-tag and T7-tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

117320531

ncbi acc num :

NP_001070962.1

ncbi gb acc num :

NM_001077494.3

uniprot acc num :

Q00653

ncbi mol weight :

38.5kDa

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Alternative NF-kappaB Pathway (138004); Cytokine Signaling In Immune System Pathway (366171); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (833822); DNA Damage Response (only ATM Dependent) Pathway (198827); EBV LMP1 Signaling Pathway (198790); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Folate Metabolism Pathway (198833)

ncbi summary :

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

uniprot summary :

NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5 -GGRNNYYCC-3 , located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. Protein type: Oncoprotein; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 10q24. Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; nucleus; cytosol. Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription factor activity. Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; follicular dendritic cell differentiation; rhythmic process; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; toll-like receptor signaling pathway; germinal center formation; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway. Disease: Immunodeficiency, Common Variable, 10

size1 :

0.01 mg

price1 :

160 USD

size2 :

0.05 mg

price2 :

320

size3 :

0.1 mg

price3 :

495

more info or order :

MyBioSource product webpage