catalog number :
MBS2009083
products type :
Recombinant Protein
products full name :
Recombinant Growth Differentiation Factor 15 (GDF15)
products short name :
Growth Differentiation Factor 15 (GDF15)
other names :
growth/differentiation factor 15; Growth/differentiation factor 15; growth/differentiation factor 15; GDF-15; macrophage inhibiting cytokine-1; growth differentiation factor 15
products gene name :
GDF15
other gene names :
Gdf15; Gdf15; SBF; MIC-1; NAG-1; Sbf; GDF-15
uniprot entry name :
GDF15_MOUSE
sequence :
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- HPRDSCPL GPGRCCHLET VQATLEDLGW SDWVLSPRQL QLSMCVGECP HLYRSANTHA QIKARLHGLQ PDKVPAPCCV PSSYTPVVLM HRTDSGVSLQ TYDDLV
form :
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: His193~Val296 (Accession # Q9Z0J7) with two N-terminal Tags, His-tag and S-tag. Predicted isoelectric point: 6.0. Subcellular Location: Secreted. Accession Number: Q9Z0J7
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_035949.2
ncbi gb acc num :
NM_011819.2
ncbi mol weight :
17.2kDa
uniprot summary :
GDF15: disulfide-linked. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted; Cytokine. Cellular Component: extracellular space; extracellular region. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: regulation of apoptosis; regulation of MAPKKK cascade; cell development