antibody

Biotin-Linked Antibody to Low Density Lipoprotein Receptor Related Protein 6 (LRP6)

MBS2008837

0.1 mg

245 USD

polyclonal

rabbit

biotin

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Antibody

Biotin-Linked Antibody to Low Density Lipoprotein Receptor Related Protein 6 (LRP6)

[Low Density Lipoprotein Receptor Related Protein 6 (LRP6)]

[low-density lipoprotein receptor-related protein 6; Low-density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6; low density lipoprotein receptor-related protein 6]

[LRP6]

[LRP6; LRP6; ADCAD2; LRP-6]

LRP6_HUMAN

Polyclonal

Rabbit

Human

Antigen-specific Affinity Chromatography

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol

200µg/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB; IHC; ICC; IP

Western blotting: 0.5-2µg/mL. Immunohistochemistry: 5-20µg/mL. Immunocytochemistry: 5-20µg/mL. Optimal working dilutions must be determined by end user

Source: Antibody labeling. Label: Biotin. Traits: Liquid. Immunogen: Recombinant LRP6 (Trp981~Gly1213) expressed in E.coli

Biotin Antibody

148727288

NP_002327.2

NM_002336.2

O75581

Canonical Wnt Signaling Pathway (138032); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); Presenilin Action In Notch And Wnt Signaling Pathway (137914); Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway And Pluripotency (198847); Wnt Signaling Network Pathway (137962); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)

This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]

LRP6: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Forms a WNT-signaling complex formed of a WNT protein, a FZD protein and LRP5 or LRP6. Interacts (via the extracellular domain) with WNT1; the interaction is enhanced by prior formation of the Wnt/Fzd complex. Interacts (via the beta-propeller regions 3 and 4) with WNT3A. Interacts (via the beta-propeller regions 1 and 2) with WNT9B. Interacts with FZD5; the interaction forms a coreceptor complex for Wnt signaling and is inhibited by DKK1 and C1orf187. Interacts (via beta propeller region) with DKK1; the interaction inhibits FZD5/LRP6 complex formation. Interacts with DKK2. Interacts with C1orf187/DRAXIN; the interaction inhibits Wnt signaling. Interacts (via the phosphorylated PPPSP motifs) with AXIN1; the interaction recruits the AXIN1/GSK3B complex to cell surface LRP6 signalsomes. Interacts with GRB10; the interaction prevents AXIN1 binding, thus negatively regulating the Wnt signaling pathway. Interacts (via the extracellular domain) with RSPO1; the interaction activates Wnt/beta-catenin signaling. Interacts (via the extracellular domain) with RSPO3 (via the cysteine rich domain); the interaction activates Wnt/beta-catenin signaling. Interacts (via the beta- propeller regions 1 and 2) with SOST; the interaction competes with DKK1 for binding for inhibiting beta-catenin signaling. Interacts with MESD; the interaction prevents the formation of LRP6 aggregates and targets LRP6 to the plasma membrane. Interacts (via the cytoplasmic domain) with CSNKIE; the interaction phosphorylates LRP6, binds AXIN1 and inhibits AXIN1/GSK3B-mediated phosphorylation of beta-catenin. Interacts with MACF1. Decreased levels on WNT3A stimulation. Widely co-expressed with LRP5 during embryogenesis and in adult tissues. Belongs to the LDLR family. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 12p13.2. Cellular Component: Golgi apparatus; cell surface; cell soma; early endosome membrane; endoplasmic reticulum; early endosome; extracellular region; plasma membrane; integral to membrane; synapse; caveola; cytoplasmic vesicle; receptor complex. Molecular Function: low-density lipoprotein receptor activity; Wnt-protein binding; identical protein binding; Wnt receptor activity; protein binding; protein homodimerization activity; frizzled binding; kinase inhibitor activity; toxin transporter activity; apolipoprotein binding; receptor binding. Biological Process: embryonic forelimb morphogenesis; response to peptide hormone stimulus; radial glial cell differentiation in the forebrain; regulation of fat cell differentiation; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; cerebellum morphogenesis; palate development; thalamus development; Wnt receptor signaling pathway through beta-catenin; embryonic pattern specification; bone remodeling; embryonic hindlimb morphogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; anterior/posterior pattern formation; synaptic transmission; elevation of cytosolic calcium ion concentration; cell-cell adhesion; negative regulation of protein amino acid phosphorylation; response to folic acid; convergent extension; midbrain development; positive regulation of mesenchymal cell proliferation; positive regulation of bone resorption; heart looping; neural crest cell differentiation; cell migration involved in gastrulation; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; external genitalia morphogenesis; Wnt receptor signaling pathway; neural crest formation; positive regulation of cell cycle; gastrulation with mouth forming second; negative regulation of fat cell differentiation; positive regulation of ossification; odontogenesis of dentine-containing teeth; formation of radial glial scaffolds; cerebral cortex cell migration; embryonic retina morphogenesis in camera-type eye; neural tube closure; midbrain-hindbrain boundary development; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; regulation of ossification. Disease: Coronary Artery Disease, Autosomal Dominant 2

0.1 mg

245 USD

0.2 mg

370

1 mg

880