catalog number :
MBS2008226
products full name :
Biotin-Linked Antibody to Mesothelin (MSLN)
products short name :
[Mesothelin (MSLN)]
other names :
[mesothelin isoform 1 preproprotein; Mesothelin; mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor]
products gene name :
[MSLN]
other gene names :
[MSLN; MSLN; MPF; SMRP; MPF; MPF]
uniprot entry name :
MSLN_HUMAN
purity :
Antigen-specific Affinity Chromatography.
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN 3 , 50% glycerol.
storage stability :
Storage : Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; ICC; IHC-P; IHC-F; IF; ELISA.
app notes :
Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
other info1 :
Source: Antibody labeling. Label: Biotin. Traits: Liquid. Immunogen: Recombinant MSLN (Glu400~Leu615) expressed in E.coli.
other info2 :
Unconjugated Antibody: The unconjugated antibody version of this item is also available as catalog #MBS2006850
products categories :
Biotin Antibody
ncbi acc num :
NP_001170826.1
ncbi gb acc num :
NM_001177355.1
ncbi summary :
This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
uniprot summary :
MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor; Cell adhesion. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane. Molecular Function: protein binding. Biological Process: pancreas development; cell adhesion