catalog number :
MBS2007658
products full name :
Biotin-Linked Antibody to Mitogen Activated Protein Kinase Kinase 4 (MAP2K4)
products short name :
Mitogen Activated Protein Kinase Kinase 4 (MAP2K4)
other names :
mitogen-activated protein kinase kinase 4; mitogen-activated protein kinase kinase 4
products gene name :
MAP2K4
other gene names :
MKK4; ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4; ATMEK4; ATMKK4; F19C24.26; F19C24_26; MAP KINASE KINASE 4; mitogen-activated protein kinase kinase 4; MKK4
sequence :
The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD WLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-VDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL MSTSFDKFYK YVYSVLDDVI PEEILGKITLATVKALNHLK ENLKIIHRDI KPSNILLDRS GNIKLCDFGI SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYELATGRFPYPKW NSVFDQLTQV VKGDPPQLSNSEEREFSPSF INFVNLCLTK DESKRPKYKELLKHPFILMY EERAVEVACY VCKILDQMPATPSS
specificity :
The antibody is a rabbit polyclonal antibody raised against MAP2K4. It has been selected for its ability to recognize MAP2K4 in immunohistochemical staining and western blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunohistochemistry (IHC) (HC-P), (IHC-F), ELISA (EIA), Western Blot (WB), ICC.
app notes :
Western blotting: 1: 1 00-400 .
Immunocytochemistry in formalin fixed celis: 1:100-500.
Immunohistochemistry in formalin fixed frozen section: 1: 1 00-500.
Immunohistochemistry in paraffin section: 1 :50-200.
Enzyme-linked Immunosorbent Assay: 1:100-200.
Optimal working dilutions must be determined by end user.
other info1 :
Accession No.: RPD564Hu01
products categories :
Biotin Antibody
ncbi acc num :
NP_175577.1
ncbi gb acc num :
NM_104044.2
ncbi pathways :
Plant-pathogen Interaction Pathway (132015); Plant-pathogen Interaction Pathway (131980)
ncbi summary :
Encodes a mitogen-activated map kinase kinase (there are nine in Arabidopsis) involved in innate immunity. This protein activates MPK3/MPK6 and early-defense genes redundantly with MKK5. In plants with both MKK5 and MKK6 levels reduced by RNAi plants, floral organs do not abscise suggestion a role for both proteins in mediating floral organ abscission.