Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) | MyBioSource MBS2007250 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

catalog :

MBS2007250

quantity :

0.05 mg

price :

300 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2007250

products type :

Antibody

products full name :

Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

products short name :

A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 7 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; COMPase; a disintegrin and metalloprotease with thrombospondin motifs-7 preproprotein; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7; ADAM metallopeptidase with thrombospondin type 1 motif, 7; COMPase

products gene name :

ADAMTS7

other gene names :

ADAMTS7; ADAMTS7; ADAM-TS7; ADAMTS-7; ADAM-TS 7; ADAM-TS 7; ADAM-TS7; ADAMTS-7

uniprot entry name :

ATS7_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rat

sequence length :

1686

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-VLPGVLY DVNHQCRLQY GPSSAYCEDV DNVCYTLWCS VGTTCHSKMD AAVDGTSCGK NKWCLNGECV PEGFQPETVD GGWSGWSAWS VCSRSCGVGV RSSERQCTQP VPKNKGKYCV GERKRYRLCN LQACP

specificity :

The antibody is a rabbit polyclonal antibody raised against ADAMTS7. It has been selected for its ability to recognize ADAMTS7 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant ADAMTS7 (Val444~Pro575) expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

38683827

ncbi acc num :

NP_055087.2

ncbi gb acc num :

NM_014272.3

uniprot acc num :

Q9UKP4

ncbi mol weight :

184,095 Da

uniprot summary :

Function: Metalloprotease that may play a role in the degradation of COMP. Ref.1. Cofactor: Binds 1 zinc ion per subunit . By similarity. Ref.1. Subunit structure: Interacts with COMP. Ref.1. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Note: Also found associated with the external cell surface . By similarity. Tissue specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments. Ref.1 Ref.4. Induction: Up-regulated in articular cartilage and synovium from arthritis patients. Ref.1. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: N-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate. Ref.4May be cleaved by a furin endopeptidase . By similarity. The precursor is sequentially processed. Ref.4. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 PLAC domain.Contains 8 TSP type-1 domains. Biophysicochemical propertiespH dependence:Optimum pH is between 7.5 and 9.5. Sequence caution: The sequence AAD56358.1 differs from that shown. Reason: Probable cloning artifact.

size :

0.05 mg

price :

300 USD

more info or order :

MyBioSource product webpage