Polyclonal Antibody to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) | MyBioSource MBS2007182 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

catalog :

MBS2007182

quantity :

0.01 mg

price :

135 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2007182

products type :

Antibody

products full name :

Polyclonal Antibody to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

products short name :

Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

other names :

myosin-7; Myosin-7; myosin-7; myHC-beta; myhc-slow; myopathy, distal 1; myosin heavy chain 7; beta-myosin heavy chain; myosin heavy chain (AA 1-96); myosin heavy chain slow isoform; rhabdomyosarcoma antigen MU-RMS-40.7A; myosin heavy chain, cardiac muscle beta isoform; myosin, heavy polypeptide 7, cardiac muscle, beta; myosin, heavy chain 7, cardiac muscle, beta; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform

products gene name :

MYH7

other gene names :

MYH7; MYH7; CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB; MYHCB; MyHC-slow; MyHC-beta

uniprot entry name :

MYH7_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

1935

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-RSV NDLTSQRAKL QTENGELSRQ LDEKEALISQ LTRGKLTYTQ QLEDLKRQLE EEVKAKNALA HALQSARHDC DLLREQYEEE TEAKAELQRV LSKANSEVAQ WRTKYETDAI QRTEELEEAK KKLAQRLQEA EEAVEAVNAK CSSLEKTKHR LQNEIEDLMV DVERSNAAAA ALDKKQRNFD KILAEWKQKY EESQSELESS QKEARSLSTE LFKLKNAYEE SLEHLETFKR ENKNLQEEIS DLTEQL

specificity :

The antibody is a rabbit polyclonal antibody raised against MYH7. It has been selected for its ability to recognize MYH7 in immunohistochemical staining and western blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

ncbi gi num :

115496169

ncbi acc num :

NP_000248.2

ncbi gb acc num :

NM_000257.2

uniprot acc num :

P12883

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Membrane Trafficking Pathway (106160); Tight Junction Pathway (83071)

ncbi summary :

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]

uniprot summary :

MYH7: Muscle contraction. Defects in MYH7 are the cause of familial hypertrophic cardiomyopathy type 1 (CMH1). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH7 are the cause of myopathy myosin storage (MYOMS). In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers. Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM); also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm. Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH7 are the cause of myopathy distal type 1 (MPD1). MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease. Protein type: Motor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 14q12. Cellular Component: nucleoplasm; sarcomere; focal adhesion; cytoplasm; stress fiber; muscle myosin complex; Z disc; myosin complex. Molecular Function: calmodulin binding; microfilament motor activity; protein binding; ATPase activity; actin-dependent ATPase activity; actin binding; ATP binding. Biological Process: adult heart development; striated muscle contraction; muscle contraction; regulation of heart rate; metabolic process; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; muscle filament sliding. Disease: Myopathy, Distal, 1; Scapuloperoneal Myopathy, Myh7-related; Myopathy, Myosin Storage; Cardiomyopathy, Dilated, 1s; Myopathy, Congenital, With Fiber-type Disproportion; Myopathy, Myosin Storage, Autosomal Recessive; Cardiomyopathy, Familial Hypertrophic, 1

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

235

size3 :

0.1 mg

price3 :

320

more info or order :

MyBioSource product webpage