Polyclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) | MyBioSource MBS2006868 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

catalog :

MBS2006868

quantity :

0.01 mg

price :

140 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2006868

products type :

Antibody

products full name :

Polyclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

products short name :

T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

other names :

T-cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain containing 9; Washington University cell adhesion molecule; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; T cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3

products gene name :

TIGIT

other gene names :

TIGIT; TIGIT; VSIG9; VSTM3; WUCAM; VSIG9; VSTM3

uniprot entry name :

TIGIT_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Homo sapiens (Human)

sequence length :

244

sequence :

The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GMMTGTIETT GNISAEKGGS IILQCHLSST TAQVTQVNWE QQDQLLAICN ADLGWHISPS FKDRVAPGPG LGLTLQSLTV NDTGEYFCIY HTYPDGTYTG RIFLEVLESS VAEHGARFQI PLLGAMAATL VVICTAVIVV VALTRKKKAL RIHSVEGDLR RKSAGQEEWS PSAPSPPGSC VQAEAAPAGL CGEQRGEDCA ELHDYFNVLS YRSL

specificity :

The antibody is a rabbit polyclonal antibody raised against TIGIT. It has been selected for its ability to recognize TIGIT in immunohistochemical staining and western blotting.

purity :

Antigen-specific Affinity Chromatography.

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

200ug/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.

app notes :

Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user

other info1 :

Immunogen: MBS2010785-Recombinant T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Source: Polyclonal antibody preparation

other info2 :

UOM: 100ug. Traits: Liquid

ncbi gi num :

256600228

ncbi acc num :

NP_776160.2

ncbi gb acc num :

NM_173799.3

uniprot acc num :

Q495A1

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

ncbi summary :

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]

uniprot summary :

TIGIT: Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 3q13.31. Cellular Component: cell surface; integral to membrane; plasma membrane. Molecular Function: protein binding; receptor binding. Biological Process: negative regulation of interleukin-12 production; negative regulation of T cell activation; positive regulation of interleukin-10 production

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

255

size3 :

0.1 mg

price3 :

345

more info or order :

MyBioSource product webpage