antibody

Biotin-Linked Antibody to Epidermal Growth Factor (EGF)

MBS2006805

0.1 mg

420 USD

polyclonal

rabbit

biotin

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Antibody

Biotin-Linked Antibody to Epidermal Growth Factor (EGF)

Epidermal Growth Factor (EGF)

epidermal growth factor; Pro-epidermal growth factor; pro-epidermal growth factor; beta-urogastrone; epidermal growth factor; Urogastrone

EGF

EGF; EGF; URG; HOMG4; EGF

EGF_HUMAN

Polyclonal

IgG

Rabbit

Porcine (Pig)

53

The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH

The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin. It has been selected for its ability to recognize EGF in immunohistochemical staining andwestern blotting.

Antigen-specific Affinity Chromatography.

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol

200ug/ml

Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB; ICC; IHC-P; IHC-F; IF; ELISA.

Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.

Source: Antibody labeling. Traits: Liquid

Immunogen: MBS2010442-Recombinant Epidermal Growth Factor (EGF)

Biotin Antibody

46242544

AAS83395.1

P01133

133,994 Da

Adaptive Immune System Pathway (366160); Arf6 Signaling Events Pathway (138034); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Ceramide Signaling Pathway (138023); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550)

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hormone; Membrane protein, integral; Ligand, receptor tyrosine kinase. Chromosomal Location of Human Ortholog: 4q25. Cellular Component: extracellular space; lysosomal membrane; integral to membrane; plasma membrane; extracellular region. Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; transmembrane receptor protein tyrosine kinase activation (dimerization); peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; innate immune response; negative regulation of secretion; angiogenesis; blood coagulation; DNA replication; positive regulation of phosphorylation; positive regulation of DNA binding; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation. Disease: Hypomagnesemia 4, Renal

0.1 mg

420 USD