catalog number :
MBS2006741
products full name :
Polyclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)
products short name :
[Cluster Of Differentiation 40 Ligand (CD40L)]
other names :
[CD40 ligand; CD40 ligand; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154]
products gene name :
[CD40L]
other gene names :
[CD40LG; CD40LG; CD40L; TNFSF5; CD40L; TNFSF5; CD40-L]
sequence :
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF- FEMHKGDQDP QIAAHVISEA SSKTASVLQW APKGYYTLST NLVTLENGRQ LAVKRQGIYY IYAQVTFCSN RDAAGQAPFI ASLCLRSPSG SERILLRAAN THSSSKPCGQ QSIHLGGVFE LQPGASVFVN VTDPSQVSHG TGFTSFGLLK L
specificity :
The antibody is a rabbit polyclonal antibody raised against CD40L. It has been selected for its ability to recognize CD40L in immunohistochemical staining andwestern blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-5000. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
other info1 :
Immunogen: CD40L, Porcine. Immunogen: Recombinant CD40L (Phe111~Leu261) with N-terminal His-Tag
expressed in E.coli. Accession No.: RPA119Po02
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2038110
ncbi acc num :
NP_999291.1
ncbi gb acc num :
NM_214126.1
ncbi mol weight :
28,869 Da
ncbi pathways :
Adaptive Immune System Pathway (1381109); Allograft Rejection Pathway (84530); Allograft Rejection Pathway (535); Asthma Pathway (84527); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84528); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (84481); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (1381218)
uniprot summary :
Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.