catalog number :
MBS2006362
products full name :
Polyclonal Antibody to Phospholipase A2, Group IID (PLA2G2D)
products short name :
Phospholipase A2, Group IID (PLA2G2D)
other names :
phospholipase A2, group IID; Group IID secretory phospholipase A2; group IID secretory phospholipase A2; GIID sPLA2; secretory phospholipase A2s; phosphatidylcholine 2-acylhydrolase 2D; secretory-type PLA, stroma-associated homolog; phospholipase A2, group IID; PLA2IID; Phosphatidylcholine 2-acylhydrolase 2D; Secretory-type PLA, stroma-associated homolog
products gene name :
PLA2G2D
other gene names :
PLA2G2D; PLA2G2D; SPLASH; sPLA2S; PLA2IID; sPLA2-IID; SPLASH; GIID sPLA2; sPLA2-IID
uniprot entry name :
PA2GD_HUMAN
specificity :
The antibody is a rabbit polyclonal antibody raised against PLA2G2D. It has been selected for its ability to recognize PLA2G2D in immunohistochemical staining and western blotting.
purity :
Antigen-specific Affinity Chromatography.
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Prodin-300, 50% glycerol.
storage stability :
Storage : Avoid repeated freeze/thaw cycles.Store at 4 degree C for frequent use.Aliquot and store at -20 degree C for 12 months. Stability Test : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition
tested application :
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
app notes :
Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.
other info1 :
Source: Polyclonal antibody preparation. Traits: Liquid
other info2 :
Immunogen: Recombinant PLA2G2D (Leu21~Cys144) expressed in E.coli.
ncbi acc num :
EAW94915.1
ncbi pathways :
Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194385)
ncbi summary :
This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
uniprot summary :
PLA2G2D: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2- linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined. Belongs to the phospholipase A2 family. Protein type: Secreted; Phospholipase; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Secreted, signal peptide; EC 3.1.1.4. Chromosomal Location of Human Ortholog: 1p36.12. Cellular Component: extracellular region. Molecular Function: phospholipase A2 activity; calcium ion binding. Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; inflammatory response; lipid catabolic process