Biotin-Linked Antibody to 5'-Nucleotidase, Ecto (NT5E) | MyBioSource MBS2006344 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Biotin-Linked Antibody to 5'-Nucleotidase, Ecto (NT5E)

catalog :

MBS2006344

quantity :

0.1 mg

price :

245 USD

clonality :

polyclonal

host :

rabbit

conjugate :

biotin

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2006344

products type :

Antibody

products full name :

Biotin-Linked Antibody to 5'-Nucleotidase, Ecto (NT5E)

products short name :

[5'-Nucleotidase, Ecto (NT5E)]

other names :

[5'-nucleotidase; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase; 5'-nucleotidase, ecto (CD73); Ecto-5'-nucleotidase]

products gene name :

[NT5E]

other gene names :

[NT5E; NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; NT5; NTE; 5'-NT]

uniprot entry name :

5NTD_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Mouse

specificity :

The antibody is a rabbit polyclonal antibody raised against NT5E conjugated to biotin. It has been selected for its ability to recognize NT5E in immunohistochemical staining andwestern blotting.

purity :

Antigen-specific Affinity Chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

200ug/ml

storage stability :

Storage : Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; ICC; IHC-P; IHC-F; IF; ELISA

app notes :

Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.

other info1 :

Source: Antibody labeling. Label: Biotin. Traits: Liquid. Immunogen: Recombinant NT5E (Trp29~phe550) expressed in E.coli.

other info2 :

Unconjugated Antibody: The unconjugated antibody version of this item is also available as catalog #MBS2002571

products categories :

Biotin Antibody

ncbi gi num :

23897

ncbi acc num :

CAA39271.1

uniprot acc num :

P21589

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (106276); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307); Pyrimidine Catabolism Pathway (106288)

ncbi summary :

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

uniprot summary :

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor; Nucleotide Metabolism - purine; Phosphatase (non-protein); Nucleotide Metabolism - pyrimidine; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5. Chromosomal Location of Human Ortholog: 6q14-q21. Cellular Component: cell surface; membrane; cytoplasm; plasma membrane. Molecular Function: 5 -nucleotidase activity; metal ion binding; nucleotide binding. Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process. Disease: Calcification Of Joints And Arteries

size1 :

0.1 mg

price1 :

245 USD

size2 :

0.2 mg

price2 :

360

size3 :

1 mg

price3 :

860

more info or order :

MyBioSource product webpage