antibody

Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

MBS2006303

0.05 mg

320 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

A disintegrin and metalloproteinase with thrombospondin motifs 12 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 12; A disintegrin and metalloproteinase with thrombospondin motifs 12; ADAM-TS12; ADAMTS-12; ADAM-TS 12; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 12; ADAM metallopeptidase with thrombospondin type 1 motif, 12

ADAMTS12

ADAMTS12; ADAMTS12; PRO4389; ADAM-TS 12; ADAM-TS12; ADAMTS-12

ATS12_HUMAN

Polyclonal

IgG

Rabbit

Mouse

1594

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-KLLY FWQFGRWTEC SVTCGTGIRR QAAHCVKKGH GIVKTTFCNP ETQPSVRQKK CHEKDCPPRW WAGEWEACST TCGPYGEKKR TVLCIQTMGS DEQALPATDC QHLLKPKALV SCNRDILCPS DWTVGNWSEC SVSCGGGVRI RSVTCAKNLN EPCDKTRKPN SRALCGLQQC P

The antibody is a rabbit polyclonal antibody raised against ADAMTS12. It has been selected for its ability to recognize ADAMTS12 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

Immunogen: Recombinant ADAMTS12 (Lys827~Pro1001) expressed in E.coli.

Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

51558724

NP_112217.2

NM_030955.2

P58397

177,676 Da

Function: Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties. Ref.5 Ref.6 Ref.7. Cofactor: Binds 1 zinc ion per subunit . By similarity. Enzyme regulation: Inhibited by alpha-2 macroglobulin. Ref.7. Subunit structure: Interacts with COMP. Ref.5. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Tissue specificity: Expressed in skeletal muscle and fat. Detected at significant levels in fetal lung. Widely expressed in gastric carcinomas and in cancer cells of diverse origin. Ref.1 Ref.5. Induction: By IFN-alpha and by IL1B/interleukin-1 beta. Up-regulated in articular cartilage and synovium from arthritis patients. Up-regulared in chondrocytes. Ref.5 Ref.7. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix . By similarity.The C-terminal four TSP1-like repeats are necessary and sufficient for binding COMP.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase.Subjected to an intracellular maturation process yielding a 120 kDa N-terminal fragment containing the metalloproteinase, disintegrin, one TSP type-1 and the Cys-rich domains and a 83 kDa C-terminal fragment containing the spacer 2 and four TSP type-1 domains.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 PLAC domain.Contains 8 TSP type-1 domains. Biophysicochemical propertiespH dependence:Optimum pH is between 7.5 and 9.5 with COMP for substrate.

0.05 mg

320 USD