catalog number :
MBS2006125
products full name :
Polyclonal Antibody to A Disintegrin And Metalloprotease 15 (ADAM15)
products short name :
A Disintegrin And Metalloprotease 15 (ADAM15)
other names :
A disintegrin and metalloproteinase with thrombospondin motifs 15 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 15; A disintegrin and metalloproteinase with thrombospondin motifs 15; ADAM-TS15; ADAMTS-15; ADAM-TS 15; metalloprotease disintegrin 15 with thrombospondin domains; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 15, preproprotein; ADAM metallopeptidase with thrombospondin type 1 motif, 15
products gene name :
ADAM15
other gene names :
ADAMTS15; ADAMTS15; ADAM-TS 15; ADAM-TS15; ADAMTS-15
uniprot entry name :
ATS15_HUMAN
sequence :
The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF- SLGDGEPCAG GQAVCMHGRC ASYAQQCQSL WGPGAQPAAP LCLQTANTRG NAFGSCGRNP SGSYVSCTPR DAICGQLQCQ TGRTQPLLGS IRDLLWETID VNGTELNCSW VHLDLGSDVA QPLLTLPGTA CGPGLVCIDH RCQRVDLLGA QECRSKCHGH GVCDSNRHCY CEEGWAPPDC TTQLKATSSL TTGLLLSLLV LLVLVMLGAS YWYRARLHQR LCQLKGPTCQ YRAAQSGPSE R
specificity :
The antibody is a rabbit polyclonal antibody raised against ADAM15. It has been selected for its ability to recognize ADAM15 in immunohistochemical staining andwestern blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200
other info1 :
Immunogen: Recombinant ADAM15 (Ser211~Arg451) expressed in E.coli.
other info2 :
Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
ncbi acc num :
NP_620686.1
ncbi gb acc num :
NM_139055.2
ncbi mol weight :
103,287 Da
ncbi summary :
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has a high sequence similarity to the proteins encoded by ADAMTS1 and ADAMTS8. [provided by RefSeq, Jul 2008]
uniprot summary :
ADAMTS15: . Protein type: Secreted, signal peptide; Protease; Motility/polarity/chemotaxis; EC 3.4.24.-; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 11q25. Cellular Component: proteinaceous extracellular matrix. Molecular Function: zinc ion binding; metalloendopeptidase activity. Biological Process: proteolysis